{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T00:02:47Z","timestamp":1777939367469,"version":"3.51.4"},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"vor","delay-in-days":120,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Science Journal"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study evaluated the effects of in ovo feeding with glycosaminoglycans and vitamins, combined with amphotericin and gentamicin, on incubation traits, intestinal morphometry, organ development, and cadherin\u20101 gene relative abundance in broilers. Fertile Ross 308 breeder eggs were randomly assigned to the following five treatments: uninjected eggs; eggs injected with a solution containing amphotericin, gentamicin, ascorbic acid, and pyridoxine; the same solution supplemented with chondroitin sulfate; glucosamine sulfate; or both glycosaminoglycans. Hatching rate, embryo mortality, and total mortality were not affected by treatments (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&gt;\u20090.05). Broilers from the solution\u2010based treatment showed higher relative heart weight at 1\u2009day of age (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). Intestinal morphometric parameters increased with age in both the jejunum and ileum. In ovo feeding modulated villus height, crypt depth, and villus height\u2013crypt depth ratio, particularly in the ileum during early posthatch development. The relative abundance of the cadherin\u20101 (CDH1) gene was influenced by the interaction between in ovo feeding and age, with higher values observed in broilers receiving glycosaminoglycan\u2010supplemented solutions at specific posthatch periods. In conclusion, in ovo feeding with glycosaminoglycans and vitamins modulated early intestinal development and relative heart weight without impairing hatchability in broilers.\n                  <\/jats:p>","DOI":"10.1111\/asj.70186","type":"journal-article","created":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T05:13:27Z","timestamp":1777698807000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects of in Ovo Feeding With Glycosaminoglycans and Vitamins on Intestinal Morphometry and Cadherin\u20101 Gene Copy Abundance in Broilers"],"prefix":"10.1111","volume":"97","author":[{"given":"Elaine Talita","family":"Santos","sequence":"first","affiliation":[{"name":"Postgraduate Program in Animal Science S\u00e3o Paulo State University  Jaboticabal S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvana Martinez","family":"Baraldi\u2010Artoni","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Animal Science S\u00e3o Paulo State University  Jaboticabal S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8547-4149","authenticated-orcid":false,"given":"Jean Kaique","family":"Valentim","sequence":"additional","affiliation":[{"name":"Department of Animal Production, Institute of Animal Science Federal Rural University of Rio de Janeiro  Serop\u00e9dica Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodrigo Gar\u00f3fallo","family":"Garcia","sequence":"additional","affiliation":[{"name":"Faculty of Agricultural Sciences Universidade Federal da Grande Dourados  Dourados Mato Grosso do Sul Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2897-4312","authenticated-orcid":false,"given":"Peter","family":"Ferket","sequence":"additional","affiliation":[{"name":"Department of Poultry Science North Carolina State University  Raleigh North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ramon Diniz","family":"Malheiros","sequence":"additional","affiliation":[{"name":"Department of Poultry Science North Carolina State University  Raleigh North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melody Martins Cavalcante","family":"Pereira","sequence":"additional","affiliation":[{"name":"Graduate Program in Animal Science Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1  Curitiba Paran\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah","family":"Sgavioli","sequence":"additional","affiliation":[{"name":"Graduate Program in Animal Science Pontif\u00edcia Universidade Cat\u00f3lica do Paran\u00e1  Curitiba Paran\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,5,1]]},"reference":[{"key":"e_1_2_9_2_1","volume-title":"Fisiologia das aves comerciais","author":"Boleli I. 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