{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T23:52:48Z","timestamp":1781826768264,"version":"3.54.5"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T00:00:00Z","timestamp":1781740800000},"content-version":"vor","delay-in-days":168,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Science Journal"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>This study investigated relationships between milk components, fatty acid (FA) composition, and days open in early lactation Holstein cows under heat stress and non\u2010heat stress conditions, and evaluated predictive ability for reproductive performance. Data from 461 multiparous cows (1155 observations) across three farms were analyzed using dairy herd improvement (DHI) records from March 2020 to September 2024. Cows were classified by days open (\u2264\u2009150 vs. \u2265151\u2009days) and season, and analyzed with linear mixed models. Cows with shorter days open consistently exhibited higher milk protein, solids\u2010not\u2010fat (SNF), and de novo FA (C4:0\u2013C14:0) within 60\u2009days in milk (DIM), while those with longer days open showed higher fat\u2010to\u2010protein ratio and preformed FA (mainly \u2265\u2009C18). These trends were more pronounced under heat stress. Receiver operating characteristic analysis was performed, and the area under the curve (AUC) values showed that SNF (AUC\u2009=\u20090.699), milk protein (AUC\u2009=\u20090.676), and de novo FA (AUC\u2009=\u20090.670) at 31 to 60 DIM under heat stress demonstrated moderate but limited discriminative ability. Farm\u2010level analysis indicated substantial inter\u2010farm and within\u2010farm variation, particularly under heat stress. Milk protein, SNF, and de novo FA may thus be potential biomarkers for prolonged days open detectable via routine DHI monitoring.<\/jats:p>","DOI":"10.1111\/asj.70203","type":"journal-article","created":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T23:20:27Z","timestamp":1781824827000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Milk Components and Fatty Acid Composition as Predictors of Days Open in Early Lactation Holstein Cows"],"prefix":"10.1111","volume":"97","author":[{"given":"Noriaki","family":"Nagahaka","sequence":"first","affiliation":[{"name":"Mie Prefectural Central Agricultural Extension Center  Matsusaka Mie Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-6559-2541","authenticated-orcid":false,"given":"Masaya","family":"Matamura","sequence":"additional","affiliation":[{"name":"Graduate School of Bioresources Mie University  Tsu Mie Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0872-3778","authenticated-orcid":false,"given":"Makoto","family":"Kondo","sequence":"additional","affiliation":[{"name":"Graduate School of Bioresources Mie University  Tsu Mie Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,18]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2019\u201016926"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2019.08.011"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022\u20100302(80)83111\u20130"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010animal\u2010031412\u2010103644"},{"key":"e_1_2_9_6_1","first-page":"260","article-title":"Influences of Nutrition and Metabolism on Fertility of Dairy Cows","volume":"9","author":"Bisinotto R. 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F.","year":"1997","journal-title":"Canadian Veterinary Journal"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3168\/jdsc.2020\u20100042"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2010\u20103707"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.18689\/ijbr\u20101000107"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1148\/radiology.143.1.7063747"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.21451\/1984\u20103143\u2010AR2019\u20100053"},{"key":"e_1_2_9_15_1","first-page":"850","article-title":"Utility of Inline Milk Fat and Protein Ratio to Diagnose Subclinical Ketosis and to Assign Propylene Glycol Treatment in Lactating Dairy Cows","volume":"56","author":"Jenkins N. T.","year":"2015","journal-title":"Canadian Veterinary Journal"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2014\u20108728"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.21451\/1984\u20103143\u2010AR992"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.1.00735"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2023\u201023723"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12711\u2010023\u201000795\u20101"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2009\u20102277"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.s0022\u20100302(93)77508\u20106"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2015\u20109840"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2009\u20102431"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3287615"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2013\u20107391"},{"key":"e_1_2_9_27_1","unstructured":"Vermunt J.J. andB.P.Tranter.2011. Heat Stress in Dairy Cattle \u2013 A Review and Some of the Potential Risks Associated With the Nutritional Management of This Condition. \u201cProceedings of the Annual Conference of the Australian Veterinary Association Queensland Division.\u201d212\u2013221."},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.s0022\u20100302(03)73803\u2010x"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2009\u20102295"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2016\u201010998"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/asj.70003"},{"key":"e_1_2_9_32_1","unstructured":"Zimbelman R.B. R. P.Rhoads M. L.Rhoads G. C.Duff L. H.Baumgard andR. J.Collier.2009. \u201cA Re\u2010evaluation of the Impact of Temperature Humidity Index (THI) and Black Globe Humidity Index (BGHI) on Milk Production in High Producing Dairy Cows.\u201d Proceedings of the Southwest Nutrition Conference Tucson Arizona. 158\u2013168."}],"container-title":["Animal Science Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/asj.70203","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/asj.70203","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/asj.70203","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T23:20:29Z","timestamp":1781824829000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/asj.70203"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":31,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1111\/asj.70203"],"URL":"https:\/\/doi.org\/10.1111\/asj.70203","archive":["Portico"],"relation":{},"ISSN":["1344-3941","1740-0929"],"issn-type":[{"value":"1344-3941","type":"print"},{"value":"1740-0929","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"2025-11-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70203"}}