{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T11:23:46Z","timestamp":1783596226113,"version":"3.55.0"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T00:00:00Z","timestamp":1783555200000},"content-version":"vor","delay-in-days":189,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003993","name":"Ministry of Agriculture, Forestry and Fisheries","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003993","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Science Journal"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Previous studies reported that nonesterified fatty acid in calcium salts has the possibility to mitigate methane emission when combined with silica powder. We investigated the effects of different lipase\u2010treated oils, combined with silica powder as an oil adsorbent, on in\u00a0vitro gas production and ruminal fermentation characteristics employing sheep ruminal fluid. In Experiment 1, treatments included five supplemental oils: palm kernel oil (PKO), palm oil (PO), sunflower oil (SFO), soybean oil (SBO), and linseed oil (LNO), supplemented levels at 2% and 4% of dry matter (DM) for each oil. Compared to the control (rolled barley substrate), supplementation with 4% DM of PKO, SBO, and LNO showed methane mitigation (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.01). In Experiment 2, PKO, SBO, and LNO were further tested in either lipase\u2010treated (NEFA) or untreated triglyceride (TG) to demonstrate effects of lipase treatment on fermentation characteristics. Although none of the TG differed in methane production, NEFA decreased methane production at 48\u2009h, DM and crude protein (CP) disappearance (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). Our findings suggest that lipase\u2010treated PKO, SBO, and LNO supplementation, when combined with silica powder, can further suppress methane production; however, it also exerts negative impacts on DM and CP disappearances.\n                  <\/jats:p>","DOI":"10.1111\/asj.70216","type":"journal-article","created":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T10:44:52Z","timestamp":1783593892000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects of Nonesterified Fatty Acids From Different Plant\u2010Derived Oils With Silica Gel Powder on In\u00a0Vitro Gas Production and Ruminal Fermentation Characteristics"],"prefix":"10.1111","volume":"97","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-5990-1559","authenticated-orcid":false,"given":"Tinglin","family":"Tsai","sequence":"first","affiliation":[{"name":"Laboratory of Animal Husbandry Resources, Division of Applied Biosciences, Graduate School of Agriculture Kyoto University  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinichi","family":"Irifune","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Husbandry Resources, Division of Applied Biosciences, Graduate School of Agriculture Kyoto University  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigeru","family":"Kikuchi","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Husbandry Resources, Division of Applied Biosciences, Graduate School of Agriculture Kyoto University  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yosuke","family":"Kishi","sequence":"additional","affiliation":[{"name":"Research and Development Department Taiyo Yushi Corp  Yokohama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isamu","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Research and Development Department Taiyo Yushi Corp  Yokohama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2576-8847","authenticated-orcid":false,"given":"Yoko","family":"Tsukahara","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Husbandry Resources, Division of Applied Biosciences, Graduate School of Agriculture Kyoto University  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9311-7432","authenticated-orcid":false,"given":"Kazato","family":"Oishi","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Husbandry Resources, Division of Applied Biosciences, Graduate School of Agriculture Kyoto University  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hajime","family":"Kumagai","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Husbandry Resources, Division of Applied Biosciences, Graduate School of Agriculture Kyoto University  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,9]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/fermentation10020110"},{"key":"e_1_2_8_3_1","volume-title":"Official Methods of Analysis of AOAC International (Vol. 17, No. 1\u20102)","author":"AOAC International","year":"2000"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/asj.13707"},{"issue":"3","key":"e_1_2_8_5_1","doi-asserted-by":"crossref","first-page":"252","DOI":"10.14710\/jitaa.49.3.252-263","article-title":"Effect of Rumen\u2010Protected Fat on In\u00a0Vitro Rumen Fermentation and Apparent Biohydrogenation of Fatty Acids","volume":"49","author":"Behan A. 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