{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T23:48:07Z","timestamp":1792021687486,"version":"4.4.0"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:00:00Z","timestamp":1791936000000},"content-version":"vor","delay-in-days":286,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Animal Science Journal"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>In this study, the productivity and post\u2010transfer pregnancy rates of bovine embryos in\u00a0vitro\u2010produced (IVP) by a two\u2010step culture system were compared with those of in\u00a0vivo\u2010developed (IVD) embryos. Ovum pick\u2010up was designated as Day 0, presumptive zygotes were cultured in modified synthetic oviduct fluid without glucose (mSOFaa), whereas late\u2010stage embryos from Day 5 onward were cultured in TCM 199 supplemented with 5% fetal calf serum and 100\u2009\u03bcM \u03b2\u2010mercaptoethanol. An average of 5.9\u2009\u00b1\u20094.8 blastocysts was obtained from 26 donors. A total of 46 fresh and 18 vitrified IVP embryos were transferred individually, resulting in 27 pregnancies (42.2%). Although the pregnancy rate of OPU\u2013IVP embryos was lower than that of IVD embryos (35\/60, 58.3%), it was within the range typically reported for commercially available IVP embryos. These findings indicate that the two\u2010step culture method enables efficient production of Japanese Black IVP embryos and is a practical and reliable method for beef cattle production in the field.<\/jats:p>","DOI":"10.1111\/asj.70247","type":"journal-article","created":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T23:05:18Z","timestamp":1792019118000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Transplantation Results of Bovine Embryos Produced by a Two\u2010Step Culture Method"],"prefix":"10.1111","volume":"97","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-9647-5207","authenticated-orcid":false,"given":"Shunpei","family":"Oguma","sequence":"first","affiliation":[{"name":"Clinic MAMA  Ogaki Gifu Imajuku Japan"},{"name":"Department of Applied Life Science Nippon Veterinary and Life Science University  Tokyo Musashino Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Tajimi","sequence":"additional","affiliation":[{"name":"Tajimi Bovine ET Clinic  Gifu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4945-0649","authenticated-orcid":false,"given":"Ryotaro","family":"Miura","sequence":"additional","affiliation":[{"name":"Department of Veterinary Nippon Veterinary and Life Science University  Tokyo Musashino Japan"},{"name":"School of Veterinary Medicine Rakuno Gakuen University  Ebetsu Hokkaido Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erina","family":"Sasaki","sequence":"additional","affiliation":[{"name":"Department of Applied Life Science Nippon Veterinary and Life Science University  Tokyo Musashino Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayaka","family":"Ishii","sequence":"additional","affiliation":[{"name":"Department of Applied Life Science Nippon Veterinary and Life Science University  Tokyo Musashino Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2141-4662","authenticated-orcid":false,"given":"Konosuke","family":"Okada","sequence":"additional","affiliation":[{"name":"Department of Applied Life Science Nippon Veterinary and Life Science University  Tokyo Musashino Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-3702-0945","authenticated-orcid":false,"given":"Kenji","family":"Furui","sequence":"additional","affiliation":[{"name":"Clinic MAMA  Ogaki Gifu Imajuku Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7593-7063","authenticated-orcid":false,"given":"Hitoshi","family":"Ushijima","sequence":"additional","affiliation":[{"name":"Department of Applied Life Science Nippon Veterinary and Life Science University  Tokyo Musashino Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,14]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1002\/(SICI)1098-2795(199907)53:3<325::AID-MRD8>3.0.CO;2-T","article-title":"Ultrastructure of Bovine Embryos Developed From in Vitro\u2010Matured and \u2010Fertilized Oocytes: Comparative Morphological Evaluation of Embryos Cultured Either in Serum\u2010Free Medium or Serum\u2010Supplemented Medium","volume":"53","author":"Abe H.","year":"1999","journal-title":"Molecular Reproduction and Development"},{"key":"e_1_2_9_3_1","first-page":"139","article-title":"Production of Dairy Cattle With Genetically Superior Using OPU Technology in Yamagata Prefecture","volume":"47","author":"Abe M.","year":"2026","journal-title":"Japanese Journal Embryo Transfer"},{"key":"e_1_2_9_4_1","first-page":"4","article-title":"Survey Results of the Pregnancy Rates in Domestic Cattle","volume":"330","author":"Artificial Insemination Association Japan (AIAJ)","year":"2026","journal-title":"Livestock Artificial Insemination"},{"key":"e_1_2_9_5_1","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/0093-691X(88)90038-6","article-title":"The Anatomy and Physiology of Pre\u2010Attachment Bovine Embryos","volume":"29","author":"Betteridge K. 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