{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T16:01:13Z","timestamp":1778342473884,"version":"3.51.4"},"reference-count":39,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,4,27]],"date-time":"2014-04-27T00:00:00Z","timestamp":1398556800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aust Veterinary J"],"published-print":{"date-parts":[[2014,5]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To evaluate three easily performed methods of skin surface sampling for bacterial culture of<jats:italic>Staphylococcus<\/jats:italic>isolates obtained from dogs with superficial bacterial pyoderma (<jats:styled-content style=\"fixed-case\">SBP<\/jats:styled-content>) presenting to two veterinary teaching hospitals in Sydney,<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>ustralia, and to determine the antimicrobial susceptibility of isolates.<\/jats:p><\/jats:sec><jats:sec><jats:title>Design<\/jats:title><jats:p>Prospective study of 27 dogs with<jats:styled-content style=\"fixed-case\">SBP<\/jats:styled-content>. Cytologically confirmed<jats:styled-content style=\"fixed-case\">SBP<\/jats:styled-content>lesions were sampled for bacterial culture using a dry cotton swab, a saline\u2010moistened cotton swab and a skin surface scraping. Isolates were identified by standard discriminatory phenotypic and biochemical analyses, and confirmed using matrix\u2010assisted laser desorption ionisation time of flight mass spectrometry (<jats:styled-content style=\"fixed-case\">MALDI\u2010TOF MS<\/jats:styled-content>). Susceptibilities to 14 antimicrobials were determined by disk diffusion and by detection of the<jats:italic>mecA<\/jats:italic>gene using<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>. Sampling methods were compared according to bacterial yield, antibiograms and bacterial phenotypic analysis. Location of causative bacteria was evaluated via 8\u2010mm punch skin biopsies using haematoxylin and eosin,<jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>ram\u2013<jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>wort and<jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>iemsa staining, and fluorescence in situ hybridisation (<jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:italic>Staphylococcus<\/jats:italic>sp. were isolated from lesions in all dogs, either<jats:italic><jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>. pseudintermedius<\/jats:italic>(24 dogs) or<jats:italic><jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>. schleiferi<\/jats:italic>(3 dogs). Susceptibility was highest to cephalexin (96%) and amoxycillin clavulanate (96%). Methicillin resistance assessed by<jats:italic>mecA<\/jats:italic>real\u2010time<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>and phenotypic oxacillin resistance was found in one dog (4.3%). Routine histology and<jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content>revealed bacteria within superficial stratum corneum.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Staphylococcal isolates from canine<jats:styled-content style=\"fixed-case\">SBP<\/jats:styled-content>demonstrated high susceptibility to common empirical antimicrobials. Histological techniques confirmed presence of bacteria at superficial sites, likely to be accessed by the sampling techniques. 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