{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T16:13:05Z","timestamp":1775059985876,"version":"3.50.1"},"reference-count":36,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2015,9,27]],"date-time":"2015-09-27T00:00:00Z","timestamp":1443312000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aust Veterinary J"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To determine the in vitro susceptibilities of koala isolates of<jats:italic>Chlamydia pecorum<\/jats:italic>to enrofloxacin and chloramphenicol, which are frequently used to treat koalas with chlamydiosis, and florfenicol, a derivative of chloramphenicol.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The in vitro susceptibilities were determined by culturing three stored isolates and seven clinical swabs of<jats:italic>C. pecorum.<\/jats:italic>Susceptibility testing was undertaken using cycloheximide\u2010treated buffalo green monkey kidney cells in 96 well microtitre plates.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The minimum inhibitory concentrations (MICs) for all isolates were 0.25\u20130.50\u2009\u00b5g\/mL (enrofloxacin), 1\u20132\u2009\u00b5g\/mL (chloramphenicol), and 1\u20132\u2009\u00b5g\/mL (florfenicol). Minimum bactericidal concentration (MBC) values for five isolates were also determined and were within one two\u2010fold dilution of MICs. The MICs and MBCs of these antimicrobials were within ranges previously reported for other chlamydial species.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>When combined with previously published pharmacokinetic data, the in vitro susceptibility results support chloramphenicol as a more appropriate treatment option than enrofloxacin for koalas with chlamydiosis. The susceptibility results also indicate florfenicol may be an appropriate treatment option for koalas with chlamydiosis, warranting further investigation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/avj.12364","type":"journal-article","created":{"date-parts":[[2015,9,28]],"date-time":"2015-09-28T03:49:38Z","timestamp":1443412178000},"page":"420-423","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["In vitro activity of chloramphenicol, florfenicol and enrofloxacin against<i>Chlamydia pecorum<\/i>isolated from koalas (<i>Phascolarctos cinereus<\/i>)"],"prefix":"10.1111","volume":"93","author":[{"given":"LA","family":"Black","sequence":"first","affiliation":[{"name":"McMaster Building B14, Faculty of Veterinary Science The University of Sydney New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"DP","family":"Higgins","sequence":"additional","affiliation":[{"name":"McMaster Building B14, Faculty of Veterinary Science The University of Sydney New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Govendir","sequence":"additional","affiliation":[{"name":"McMaster Building B14, Faculty of Veterinary Science The University of Sydney New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,9,27]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(98)00302-2"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0723-2020(96)80077-4"},{"key":"e_1_2_7_4_1","unstructured":"GriffithJE.Studies into the diagnosis treatment and management of chlamydiosis in koalas. 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