{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T13:02:18Z","timestamp":1762606938157},"reference-count":12,"publisher":"Wiley","issue":"1-2","license":[{"start":{"date-parts":[[2017,1,26]],"date-time":"2017-01-26T00:00:00Z","timestamp":1485388800000},"content-version":"vor","delay-in-days":25,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aust Veterinary J"],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>The potential tissue replication sites and specific cell types that support in vivo virus survival beyond the acute phase of bovine ephemeral fever virus (<jats:styled-content style=\"fixed-case\">BEFV<\/jats:styled-content>) infection have not been fully defined in cattle. To clarify the knowledge gap, tissue specimens were tested after collection from an adult steer necropsied 1 week after acute <jats:styled-content style=\"fixed-case\">BEF<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Case report<\/jats:title><jats:p>Significant necropsy findings included fibrinoproliferative synovitis in the stifle joints and fibrin clot\u2010laden fluid in serous body cavities. Moderate numbers of infiltrating neutrophils were demonstrated in sections of the prefemoral lymph nodes and haemal node, and lymphoid hyperplasia in the spleen, haemal node and prefemoral lymph nodes. Viral <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> was detected by <jats:styled-content style=\"fixed-case\">qRT\u2010PCR<\/jats:styled-content> in fresh spleen, haemal node, prefemoral lymph node, synovial fluid and in several spleen\u2010derived cell cultures. <jats:styled-content style=\"fixed-case\">BEFV<\/jats:styled-content> was isolated from autogenously derived splenic primary cell cultures 6\u2009days after cessation of viraemia, and characteristic bullet\u2010shaped virions were confirmed by electron microscopy of an ultrathin haemal node section. In sections of the spleen, haemal node and other tissues, immunohistochemistry demonstrated <jats:styled-content style=\"fixed-case\">BEFV<\/jats:styled-content> antigens that were intracellularly associated with probable histiocytic cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>\n<jats:styled-content style=\"fixed-case\">BEFV<\/jats:styled-content> has preferential tropism for bovine lymphoid tissues and the spleen and haemal node may be potential sites for post\u2010viraemic virus replication.<\/jats:p><\/jats:sec>","DOI":"10.1111\/avj.12551","type":"journal-article","created":{"date-parts":[[2017,1,26]],"date-time":"2017-01-26T05:50:21Z","timestamp":1485409821000},"page":"49-52","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Post\u2010viraemic detection of bovine ephemeral fever virus by use of autogenous lymphoid tissue\u2010derived bovine primary cell cultures"],"prefix":"10.1111","volume":"95","author":[{"given":"R","family":"Barigye","sequence":"first","affiliation":[{"name":"Berrimah Veterinary Laboratories, Department of Primary Industry &amp; Fisheries  Darwin Northern Territory, Australia"},{"name":"Department of Veterinary Medicine, College of Food &amp; Agriculture United Arab Emirates University  Al Ain UAE"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Davis","sequence":"additional","affiliation":[{"name":"Berrimah Veterinary Laboratories, Department of Primary Industry &amp; Fisheries  Darwin Northern Territory, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Hunt","sequence":"additional","affiliation":[{"name":"Berrimah Veterinary Laboratories, Department of Primary Industry &amp; Fisheries  Darwin Northern Territory, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N","family":"Hunt","sequence":"additional","affiliation":[{"name":"Berrimah Veterinary Laboratories, Department of Primary Industry &amp; Fisheries  Darwin Northern Territory, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Walsh","sequence":"additional","affiliation":[{"name":"Berrimah Veterinary Laboratories, Department of Primary Industry &amp; 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