{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,9]],"date-time":"2025-12-09T08:23:18Z","timestamp":1765268598680,"version":"3.40.5"},"reference-count":15,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2020,7,8]],"date-time":"2020-07-08T00:00:00Z","timestamp":1594166400000},"content-version":"am","delay-in-days":366,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2019,7,8]],"date-time":"2019-07-08T00:00:00Z","timestamp":1562544000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"publisher","award":["LP160101599"],"award-info":[{"award-number":["LP160101599"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aust Veterinary J"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:p>Infectious Ovine Keratoconjunctivitis (IOK) is a contagious ocular disease of sheep. A range of organisms have been observed as the aetiological agents of IOK. In this study, the presence of chlamydial pathogens (<jats:italic>C. pecorum, C. abortus, C. psittaci<\/jats:italic>) in conjunctival swabs was tested for. The swabs were collected from sheep with varying grades of IOK in an Australian pre\u2010export feedlot. The sheep had been rejected from a shipment because of the eye disease. The relative contribution of chlamydial pathogens to IOK and the rejection of animals was evaluated.<\/jats:p><jats:p>In total, 149 conjunctival swabs were taken from rejected sheep (IOK Grades 1 to 6; n = 126) as well as those with healthy eyes (Grade 0; n = 23). Screening for chlamydial pathogens was done using species\u2013specific qPCR assays. Chlamydial DNA was detected in 35.6% (53\/149) of conjunctival samples. <jats:italic>C. pecorum<\/jats:italic> was the most predominant species with an overall prevalence of 28.9% (43\/149). <jats:italic>C. psittaci<\/jats:italic> prevalence was 6.7% (10\/149). Both organisms were detected in healthy as well as IOK\u2010affected eyes. All swabs tested negative for <jats:italic>C. abortus<\/jats:italic>.<\/jats:p><jats:p>The results from this study demonstrate that <jats:italic>Chlamydia<\/jats:italic> spp can be readily detected in sheep presenting with IOK. The zoonotic <jats:italic>C. abortus<\/jats:italic> was not detected in any of the samples in this study, providing further evidence to the suggestion that this pathogen remains absent from Australia. Although the exact contribution of <jats:italic>Chlamydia<\/jats:italic> spp in the IOK pathogenesis is unclear, such studies are anticipated to be of benefit to Australian domestic and live export production systems.<\/jats:p>","DOI":"10.1111\/avj.12857","type":"journal-article","created":{"date-parts":[[2019,7,9]],"date-time":"2019-07-09T04:52:48Z","timestamp":1562647968000},"page":"401-403","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Detection of <i>Chlamydiaceae<\/i> in ocular swabs from Australian pre\u2010export feedlot sheep"],"prefix":"10.1111","volume":"97","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1340-2562","authenticated-orcid":false,"given":"M","family":"Jelocnik","sequence":"first","affiliation":[{"name":"Animal Research Centre University of the Sunshine Coast  Sippy Downs Queensland 4556 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Laurence","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, College of Science, Health, Education and Engineering Murdoch University  Western Australia, 6150"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"FR","family":"Murdoch","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, College of Science, Health, Education and Engineering Murdoch University  Western Australia, 6150"},{"name":"Farm Animal Practice, Royal (Dick) School of Veterinary Studies University of Edinburgh, Easter Bush Campus  Roslin United Kingdom, EH25 9RG"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Polkinghorne","sequence":"additional","affiliation":[{"name":"Animal Research Centre University of the Sunshine Coast  Sippy Downs Queensland 4556 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,7,8]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.125.10.253"},{"key":"e_1_2_8_3_1","unstructured":"ChapmanHM MurdochFR RobertsonID et al. Detection identification and treatment of ovine keratoconjunctivitis at a Western Australian pre embarkation feedlot. 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