{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T19:09:39Z","timestamp":1790104179505,"version":"4.0.1"},"reference-count":33,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2019,7,8]],"date-time":"2019-07-08T00:00:00Z","timestamp":1562544000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000982","name":"Rural Industries Research and Development Corporation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000982","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aust Veterinary J"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To investigate the relationship between bronchoalveolar lavage fluid cytology, particularly mast cells, and airway hyper\u2010reactivity in athletic horses presented for poor performance that included a respiratory tract evaluation in two disparate locations in Australia.<\/jats:p><\/jats:sec><jats:sec><jats:title>Design<\/jats:title><jats:p>Multi\u2010centre, retrospective and prospective cross\u2010sectional study<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Eighty four adult horses underwent both pulmonary function testing and histamine bronchoprovocation with a commercial flowmetric plethysmography system. A bronchoalveolar lavage was performed four to twelve hours later. Bronchoalveolar lavage fluid cytology was categorised using two differing classification systems to define mild equine asthma. Statistical analysis was used to assess associations between bronchoalveolar lavage fluid relative inflammatory cell percentages, and airway hyper\u2010reactivity and their associated categorisations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Sixty four percent (54\/84) of horses displayed airway hyper\u2010reactivity, as defined by PC<jats:sub>35<\/jats:sub>\u2009&lt;\u20096\u2009mg\/ml of histamine. A relative mastocytosis was the most common bronchoalveolar lavage fluid cytological abnormality. Horses with a sole mast cell response of \u2265 5% within their bronchoalveolar lavage fluid displayed airway hyper\u2010reactivity at a lower dose of nebulized histamine than horses with normal bronchoalveolar lavage fluid cytology. Horses with mixed cell responses (relative mast cell percentage\u2009&gt;\u20092% and\/or relative neutrophil percentage\u2009&gt;\u20095% and\/or eosinophil relative cell percentage\u2009\u2265\u20091%) displayed airway hyper\u2010reactivity at a lower dose of nebulized histamine than horses with normal bronchoalveolar lavage fluid cytology.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In the Australian context, recently revised increased bronchoalveolar lavage fluid cytology relative cell percentage cut offs appear appropriate for sole mast cell responses. The historical lower cut offs appear to be appropriate for mixed inflammatory cell responses.<\/jats:p><\/jats:sec>","DOI":"10.1111\/avj.12861","type":"journal-article","created":{"date-parts":[[2019,7,9]],"date-time":"2019-07-09T06:24:30Z","timestamp":1562653470000},"page":"343-350","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["The relationship between bronchoalveolar lavage fluid cytology and airway hyper\u2010reactivity in a population of Australian horses presented for poor performance"],"prefix":"10.1111","volume":"97","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2268-1452","authenticated-orcid":false,"given":"CJ","family":"Secombe","sequence":"first","affiliation":[{"name":"School of Veterinary Medicine, College of Science, Health, Engineering and Education Murdoch University, 90 South Street  Murdoch Western Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"AW","family":"van Eps","sequence":"additional","affiliation":[{"name":"Equine Specialist Hospital, School of Veterinary Science The University of Queensland  Gatton Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Bruce","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, College of Science, Health, Engineering and Education Murdoch University, 90 South Street  Murdoch Western Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"GD","family":"Lester","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, College of Science, Health, Engineering and Education Murdoch University, 90 South Street  Murdoch Western Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,7,8]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.2006.tb05599.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2009.04.016"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.242.8.1138"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/evj.12482"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.15226"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.13824"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3292.1999.tb01564.x"},{"key":"e_1_2_7_9_1","first-page":"52","volume-title":"Inflammatory Airway Disease: Defining the Syndrome","author":"Viel L","year":"2002"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2007.tb02975.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2010.11.018"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2011.00847.x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/avj.12315"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.12333"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/eve.13112"},{"key":"e_1_2_7_16_1","first-page":"176","article-title":"Association between bronchoalveolar lavage cytologic features and airway reactivity in horses with a history of exercise intolerance","volume":"59","author":"Hoffman AM","year":"1998","journal-title":"Am J Vet Res"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.2001.62.1812"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2008.0109.x"},{"key":"e_1_2_7_19_1","first-page":"92","article-title":"Airway mucus in recurrent airway obstruction\u2010short term response to environmental challenge","volume":"18","author":"Gerber V","year":"2004","journal-title":"J Vet Intern Med"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cveq.2008.04.003"},{"key":"e_1_2_7_21_1","unstructured":"Ambulatory Monitoring Inc. 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