{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T04:37:23Z","timestamp":1792211843966,"version":"4.4.0"},"reference-count":41,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2021,7,13]],"date-time":"2021-07-13T00:00:00Z","timestamp":1626134400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aust Veterinary J"],"published-print":{"date-parts":[[2021,10]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>This study investigated the effect of melatonin administration in combination with the CoSynch\u201056 protocol on the conception rate after artificial insemination (AI) in beef heifers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Eighty\u2010six beef heifers were allocated to four treatments in combination with CoSynch\u201056 treatment. All heifers, excluding the control group (CTR\u00a0=\u00a025), were injected with melatonin on day 1. The melatonin (MT\u00a0=\u00a020) and MT\u2009+\u2009human chorionic gonadotropin (MT\u2009+\u2009hCG\u00a0=\u00a021) groups received no further treatment with melatonin. Each heifer was treated with gonadotropin\u2010releasing hormone (GnRH) on day 4, prostaglandin F2\u03b1 (PGF2\u03b1) on day 11, and GnRH and AI 56\u2009h later. The fourth group (2MT\u00a0=\u00a020) was injected again with melatonin concurrent with AI, and the MT\u2009+\u2009hCG group received hCG on day 19. Pregnancy diagnosis was performed using transrectal ultrasonography 45\u2009days after AI and blood samples were collected via caudal venipuncture on days 0\u20131, 14\u201315, 24 and 59. Concentrations of progesterone (P4) and the activities of oxidative stress\u2010related enzymes were determined using enzyme\u2010linked immunosorbent assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The conception rate was greater in the 2MT group (75%) than in the other groups, while there was no significant difference among the CTR (44%), MT (45%) and MT\u2009+\u2009hCG (38.1%) groups. Treatment with melatonin increased superoxide dismutase and glutathione peroxidase activities and decreased malondialdehyde concentrations but did not significantly affect the concentration of P4.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our results indicate that the administration of melatonin twice during the CoSynch\u201056 protocol may increase conception rates in beef heifers.<\/jats:p><\/jats:sec>","DOI":"10.1111\/avj.13100","type":"journal-article","created":{"date-parts":[[2021,7,13]],"date-time":"2021-07-13T08:42:32Z","timestamp":1626165752000},"page":"449-455","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Two melatonin treatments improve the conception rate after fixed\u2010time artificial insemination in beef heifers following synchronisation of oestrous cycles using the <scp>CoSynch<\/scp>\u201056 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