{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:43:04Z","timestamp":1747183384719,"version":"3.40.5"},"reference-count":47,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,2,2]],"date-time":"2022-02-02T00:00:00Z","timestamp":1643760000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aust Veterinary J"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To investigate if modification of a gonadotrophin\u2010releasing hormone (GnRH) based protocol to synchronise oestrus in <jats:italic>Bos indicus<\/jats:italic> cattle will improve response rates to the first administration of GnRH and improve pregnancy rates to artificial insemination (AI).<\/jats:p><\/jats:sec><jats:sec><jats:title>Design<\/jats:title><jats:p>Randomised control study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:italic>Bos indicus<\/jats:italic> heifers, nonlactating and lactating cows allocated to a GnRH\u201018 treatment (n\u00a0=\u00a0237) were treated with an intravaginal device (IVD) and cloprostenol (0.5\u2009mg IM) on day \u221211 and on day 0 remaining animals in the GnRH\u20107 treatment (n\u00a0=\u00a0222) were administered an IVD and every animal was treated with GnRH (100\u2009\u03bcg IM). On day 7, equine chorionic gonadotrophin (400\u2009IU IM) and cloprostenol were administered and IVD's were removed. Animals detected in oestrus on day 9 were artificially inseminated while those not detected in oestrus were administered GnRH (100\u2009\u03bcg IM) at 1700\u2009hours and inseminated on day 10. Bulls were inserted 2\u2009weeks after completion of AI and remained until day 65.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The GnRH\u201018 protocol increased the diameter of the largest follicle in the ovary on day 0, increased the percentage of new CL's induced after day 0 (46.3% vs 36.1%, for GnRH\u201018 and GnRH\u20107; P\u00a0=\u00a00.022), decreased circulating concentrations of progesterone on day 7 and increased odds of pregnancy to AI in heifers but not in nonlactating and lactating cows.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Treatment with the GnRH\u201018 compared to the GnRH\u20107 protocol increased pregnancy rates to AI in heifers but not in nonlactating or lactating 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