{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T02:07:27Z","timestamp":1790129247078,"version":"4.0.1"},"reference-count":32,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2022,5,18]],"date-time":"2022-05-18T00:00:00Z","timestamp":1652832000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aust Veterinary J"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:p>Multiple ovulation and embryo transfer (MOET) technologies are integral to genetic improvement programs in the sheep industries. Despite the protocols being well established, previous findings regarding the effects of embryo properties on MOET success remain contradictory. The objective of this study was to determine the effects of embryo developmental stage and quality on embryo viability following transfer to recipient ewes. Data including details of 377 embryos collected from 45 Merino donor ewes were obtained from MOET trials conducted on three separate farms on day 6 after laparoscopic artificial insemination (AI). A total of 270 embryos were classified as being of transferrable grade (grade 1: n\u00a0=\u00a0233; grade 2: n\u00a0=\u00a037). One or two transferrable grade embryos were transferred to each of 256 synchronised recipient ewes and pregnancy diagnosis was performed on day 36 after embryo transfer. Embryos at the hatched blastocyst stage tended to have greater viability in vivo compared to embryos at the late morula stage (59.0\u2009\u00b1\u200910.6% vs. 36.2\u2009\u00b1\u20099.7%; P\u00a0=\u00a00.083). The viability of grade 1 embryos was greater than that of grade 2 embryos (53.6\u2009\u00b1\u20097.8% vs. 35.9\u2009\u00b1\u200910.2%; P\u2009&lt;\u20090.05). The results suggest that the success of the MOET trials was influenced by the transfer of embryos at the late morula stage, almost half of which were classified as grade 2 embryos. These findings highlight the importance of following strict embryo quality grading criteria to inform the most economical management of recipient ewes and maximize pregnancy outcomes.<\/jats:p>","DOI":"10.1111\/avj.13174","type":"journal-article","created":{"date-parts":[[2022,5,18]],"date-time":"2022-05-18T07:20:06Z","timestamp":1652858406000},"page":"451-458","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Multiple ovulation and embryo transfer in sheep: Effects of embryo developmental stage and quality on viability in vivo under farm conditions"],"prefix":"10.1111","volume":"100","author":[{"given":"CAF","family":"King","sequence":"first","affiliation":[{"name":"Sydney School of Veterinary Science, Faculty of Science The University of Sydney  Camden New South Wales 2570 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