{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T21:36:09Z","timestamp":1792445769659,"version":"4.5.0"},"reference-count":31,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2023,8,3]],"date-time":"2023-08-03T00:00:00Z","timestamp":1691020800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aust Veterinary J"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:p>Hookworms (Ancylostomatidae) are well\u2010known parasites in dogs due to their health impacts and zoonotic potential. While faecal analysis is the traditional method for detection, improvements in husbandry and deworming have decreased their prevalence in urban owned dogs. Drug resistance in<jats:italic>Ancylostoma caninum<\/jats:italic>is becoming a discussion point in small animal practices across the region. This study aimed to identify hookworm species present in Australian and New Zealand dogs using molecular techniques. The ITS\u20102 and isotype\u20101 \u03b2\u2010tubulin assays were used to identify and quantify hookworm species. Results showed absence of coinfection in Australian samples from Greater Sydney region belonging either to<jats:italic>A. caninum<\/jats:italic>or<jats:italic>Uncinaria stenocephala<\/jats:italic>, while New Zealand samples were a mixture of<jats:italic>A. caninum<\/jats:italic>and<jats:italic>U. stenocephala<\/jats:italic>. The amplified isotype\u20101 \u03b2\u2010tubulin sequences exhibited susceptibility to benzimidazole drugs. Rare mutations were identified in<jats:italic>A. caninum<\/jats:italic>and<jats:italic>U. stenocephala<\/jats:italic>sequences, representing a small percentage of reads. This study highlights the importance of molecular techniques in accurately identifying and quantifying hookworm species in dog populations.<\/jats:p>","DOI":"10.1111\/avj.13272","type":"journal-article","created":{"date-parts":[[2023,8,4]],"date-time":"2023-08-04T05:48:46Z","timestamp":1691128126000},"page":"373-376","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Unambiguous identification of<i>Ancylostoma caninum<\/i>and<i>Uncinaria stenocephala<\/i>in Australian and New Zealand dogs from faecal samples"],"prefix":"10.1111","volume":"101","author":[{"given":"T","family":"Stocker","sequence":"first","affiliation":[{"name":"Sydney School of Veterinary Science, Faculty of Science The University of Sydney Sydney New South Wales 2006 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I","family":"Scott","sequence":"additional","affiliation":[{"name":"School of Veterinary Science Massey 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