{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T15:26:45Z","timestamp":1788276405901,"version":"build-2803163510"},"reference-count":23,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T00:00:00Z","timestamp":1782345600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T00:00:00Z","timestamp":1782345600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aust Veterinary J"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Asymptomatic thrombocytopenia, often associated with macroplatelets, is an important clinical entity that may be misdiagnosed as immune thrombocytopenia due to analyser limitations and low clinician awareness. It is well recognised in Cavalier King Charles Spaniels and other breeds, and has been reported in Akitas in the United States. However, its occurrence and significance in Australian Akitas remain unknown.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>Eighteen clinically healthy Akitas and 11 control dogs from Eastern Australia were evaluated. Blood samples were analysed using an automated haematology analyser, with manual platelet counts and blood film examination performed for comparison. Descriptive and non\u2010parametric statistical analyses were undertaken.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      None of the dogs had clinical signs of bleeding. Thrombocytopenia (&lt;150\u2009\u00d7\u200910\n                      <jats:sup>9<\/jats:sup>\n                      \/L) was identified in 83% of Akitas, while all controls had platelet counts above this threshold. Automated platelet counts were significantly lower than manual counts in Akitas, but not in controls. Macroplatelets were observed in a minority of dogs (11% of Akitas, 9% of controls). Mean platelet volume was significantly higher in Akitas. Additionally, Akitas had lower mean corpuscular volume, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration and reticulocyte haemoglobin equivalent than controls, and an increased red cell distribution width consistent with anisocytosis. American Akitas had a lower mean corpuscular volume than Japanese Akitas.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Asymptomatic thrombocytopenia was common in Australian Akitas but was not consistently associated with macroplatelets. Discrepancies between automated and manual platelet counts highlight the risk of underestimation by analysers. The condition occurred in both Japanese and American Akita lines without major platelet differences. Awareness and manual confirmation are essential to avoid misdiagnosis and unnecessary treatment.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/avj.70111","type":"journal-article","created":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T16:03:36Z","timestamp":1782403416000},"page":"600-604","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Asymptomatic thrombocytopenia in Akitas: a prospective cross\u2010sectional study of platelet characteristics in an Eastern Australian population"],"prefix":"10.1111","volume":"104","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-8668-5093","authenticated-orcid":false,"given":"JD","family":"Bool","sequence":"first","affiliation":[{"name":"The Pet Specialists  Terrey Hills New South Wales 2084 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1754-500X","authenticated-orcid":false,"given":"SM","family":"Jaensch","sequence":"additional","affiliation":[{"name":"Vetnostics  Macquarie Park New South Wales 2113 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"Collins","sequence":"additional","affiliation":[{"name":"The Pet Specialists  Terrey Hills New South Wales 2084 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"Thrift","sequence":"additional","affiliation":[{"name":"The Pet Specialists  Terrey Hills New South Wales 2084 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,25]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvsm.2022.07.005"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.135.12.281"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2012.00455.x"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/0192623311416261"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2011.00320.x"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12331"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-5827.1994.tb03921.x"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.c4782"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12168"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-0813.2005.tb13055.x"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2008.0085.x"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2002.tb02350.x"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-4431.2011.00702.x"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12370"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.59.495"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12112"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.2754\/avb201786040385"},{"key":"e_1_2_9_19_1","first-page":"511","article-title":"Genetic, hematologic abnormalities of Japanese Akita dogs: microcytosism anemia, na\u2010pump persistence, and macrothrombocytopenia \u2013 compensatory effects of na\u2010pump heterozygosity","volume":"49","author":"Fotea A","year":"2020","journal-title":"Vet Clin Pathol"},{"key":"e_1_2_9_20_1","unstructured":"Australian National Kennel Council.Breeds Akita. 2019. Available at:www.dogsnsw.org.au. Accessed 4 January 2025."},{"key":"e_1_2_9_21_1","unstructured":"Dogs Australia.National animal registration analysis 2018\u20132024. Dogs Australia 2024. Available at:https:\/\/dogsaustralia.org.au\/media\/10305\/rego-stats-list_2018-2024.pdf. 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