{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T06:54:41Z","timestamp":1790924081057,"version":"4.1.0"},"reference-count":50,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T00:00:00Z","timestamp":1784592000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T00:00:00Z","timestamp":1784592000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aust Veterinary J"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:p>\n                    Antimicrobial resistance (AMR) in\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    is a growing concern in both human and veterinary medicine, driven by the inappropriate use of antimicrobials. Companion animals may act as community reservoirs and sentinels for antimicrobial\u2010resistant bacteria due to their close contact with humans and other animals. However, resistance carriage in urban stray cats is poorly defined, particularly as many have limited direct antimicrobial exposure. This population provides an opportunity to assess baseline antimicrobial resistance carriage in companion animals with relatively low selection pressure. This study aimed to investigate the carriage of antimicrobial\u2010resistant\n                    <jats:italic>E. coli<\/jats:italic>\n                    in the stray cat population in Perth, Western Australia. A total of 72 faecal samples from urban stray cats (May 2024\u2013November 2024) were screened for carriage of antimicrobial\u2010resistant\n                    <jats:italic>E. coli<\/jats:italic>\n                    . Chromogenic selective agars infused with antimicrobials were used to detect resistance to ampicillin (first line) and critically important antimicrobials (CIA), including ciprofloxacin and extended\u2010spectrum cephalosporins. Presumptive\n                    <jats:italic>E. coli<\/jats:italic>\n                    were confirmed by matrix\u2010assisted laser desorption\/ionization time\u2010of\u2010flight mass spectrometry (MALDI\u2010TOF MS) and tested by broth microdilution using the Robotic Antimicrobial Susceptibility Platform (RASP). Screening of 72 faecal samples yielded\n                    <jats:italic>E. coli<\/jats:italic>\n                    growth from 69 samples (95.8%). Of these, 37.7% (26\/69) carried ampicillin\u2010resistant\n                    <jats:italic>E. coli<\/jats:italic>\n                    , 1.4% (1\/69) exhibited an extended\u2010spectrum beta\u2010lactamases phenotype, and no ciprofloxacin\u2010resistant\n                    <jats:italic>E. coli<\/jats:italic>\n                    were detected. A total of 27\n                    <jats:italic>E. coli<\/jats:italic>\n                    isolates underwent MIC testing, showing resistance to ampicillin (100%), cefotaxime (7.4%), ceftazidime (7.4%), sulfamethoxazole (33.3%), tetracycline (37.0%), and trimethoprim (29.6%). Ampicillin\u2010resistant\n                    <jats:italic>E. coli<\/jats:italic>\n                    was common among urban stray cats, while no ciprofloxacin resistance was detected, suggesting low immediate CIA\u2010related risk. However, the presence of extended\u2010spectrum cephalosporin resistance, albeit at low levels, warrants further investigation given the potential for transmission and dissemination via mobile genetic elements. These findings indicate that urban stray cats can carry and potentially spread resistant bacteria despite their limited opportunities for antimicrobial exposure.\n                  <\/jats:p>","DOI":"10.1111\/avj.70118","type":"journal-article","created":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T13:29:52Z","timestamp":1784640592000},"page":"681-688","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Carriage of antimicrobial\u2010resistant\n                    <i>E. coli<\/i>\n                    in urban stray cats (\n                    <i>Felis catus<\/i>\n                    <i>)<\/i>"],"prefix":"10.1111","volume":"104","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-4520-3023","authenticated-orcid":false,"given":"JS","family":"Kuan","sequence":"first","affiliation":[{"name":"School of Veterinary Medicine Murdoch University  Perth Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Mukerji","sequence":"additional","affiliation":[{"name":"School of Medical, Molecular &amp; Forensic Sciences Murdoch University  Perth Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7729-0427","authenticated-orcid":false,"given":"D","family":"Hampson","sequence":"additional","affiliation":[{"name":"School of Medical, Molecular &amp; Forensic Sciences Murdoch University  Perth Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Abraham","sequence":"additional","affiliation":[{"name":"School of Medical, Molecular &amp; Forensic Sciences Murdoch University  Perth Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,21]]},"reference":[{"issue":"3","key":"e_1_2_8_2_1","article-title":"The use of antibiotics and antimicrobial resistance in veterinary medicine, a complex phenomenon: a narrative review","volume":"12","author":"Caneschi A","year":"2023","journal-title":"Antibiotics (Basel, Switzerland)"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens12091074"},{"key":"e_1_2_8_4_1","unstructured":"MorrisonBJ.E. coliin cats [Internet].2023. [cited 2024 Aug 18]. Available from:https:\/\/www.petmd.com\/cat\/general-health\/e-coli-in-cats."},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.ARBA-0026-2017"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/jsap.12525"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0277664"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.02872"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.101070"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1071\/wr14180"},{"key":"e_1_2_8_11_1","unstructured":"Fact sheet: The impact of cats in Australia [Internet]. Threatened Species Recovery Hub National Environmental Science Program.2020. [cited 2025 Feb 14]. Available from:https:\/\/invasives.org.au\/publications\/fact-sheet-impact-of-cats-in-australia\/."},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci9110631"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics11060828"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sjbs.2023.103786"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-021-02810-3"},{"key":"e_1_2_8_16_1","unstructured":"National Centre for Antimicrobial Stewardship [Internet].Ncas-australia.org2024. [cited 2024 Aug 18]. Available from:https:\/\/www.ncas-australia.org\/one-health#:~:text=This%20approach%20utilises%20multidisciplinary%20expertise."},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.23.12.0695"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/metabo13080958"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/cpz1.20"},{"issue":"2","key":"e_1_2_8_20_1","first-page":"73","article-title":"Research concerning use of long\u2010term preservation techniques for microorganisms","volume":"47","author":"Criste A","year":"2014","journal-title":"Sci Pap Anim Sci Biotechnol"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/gbe\/evw054"},{"key":"e_1_2_8_22_1","unstructured":"Storing bacterial samples for optimal viability. [Internet]. [cited 2024 Oct 22]. 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