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This is accompanied by an enhanced risk of intra\u2010uterine and perinatal complications. High concentrations of sulphated progesterone metabolites (PMS<\/jats:styled-content>) have been suggested to be involved in the multifactorial aetiopathogenesis of ICP<\/jats:styled-content>. The aim of this study was to investigate further the mechanism accounting for the beneficial effect of oral administration of ursodeoxycholic acid (UDCA<\/jats:styled-content>), which is the standard treatment, regarding bile acid and PMS<\/jats:styled-content> homeostasis in the mother\u2010placenta\u2010foetus trio.<\/jats:p><\/jats:sec>Method<\/jats:title>Using HPLC<\/jats:styled-content>\u2010MS<\/jats:styled-content>\/MS<\/jats:styled-content> bile acids and PMS<\/jats:styled-content> were determined in maternal and foetal serum and placenta. The expression of ABC<\/jats:styled-content> proteins in placenta was determined by real time quantitative PCR (RT<\/jats:styled-content>\u2010QPCR<\/jats:styled-content>) and immunofluorescence.<\/jats:p><\/jats:sec>Results<\/jats:title>In ICP<\/jats:styled-content>, markedly increased concentrations of bile acids (tauroconjugates > glycoconjugates >> unconjugated), progesterone and PMS<\/jats:styled-content> in placenta and maternal serum were accompanied by enhanced concentrations in foetal serum of bile acids, but not of PMS<\/jats:styled-content>. UDCA<\/jats:styled-content> treatment reduced bile acid accumulation in the mother\u2010placenta\u2010foetus trio, but had no significant effect on progesterone and PMS<\/jats:styled-content> concentrations. ABCG<\/jats:styled-content>2<\/jats:italic>\u2005mRNA<\/jats:styled-content> abundance was increased in placentas from ICP<\/jats:styled-content> patients vs.<\/jats:italic> controls and remained stable following UDCA<\/jats:styled-content> treatment, despite an apparent further increase in ABCG<\/jats:styled-content>2.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>UDCA<\/jats:styled-content> administration partially reduces ICP<\/jats:styled-content>\u2010induced bile acid accumulation in mothers and foetuses despite the lack of effect on concentrations of progesterone and PMS<\/jats:styled-content> in maternal serum. Up\u2010regulation of placental ABCG<\/jats:styled-content>2 may play an important role in protecting the foetus from high concentrations of bile acids and PMS<\/jats:styled-content> during ICP<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/bcp.12480","type":"journal-article","created":{"date-parts":[[2014,8,7]],"date-time":"2014-08-07T10:39:26Z","timestamp":1407407966000},"page":"316-329","source":"Crossref","is-referenced-by-count":50,"title":["Effect of ursodeoxycholic acid treatment on the altered progesterone and bile acid homeostasis in the mother\u2010placenta\u2010foetus trio during cholestasis of pregnancy"],"prefix":"10.1111","volume":"79","author":[{"given":"Maria C.","family":"Esti\u00fa","sequence":"first","affiliation":[{"name":"Ram\u00f3n Sard\u00e1 Mother' and Children's Hospital Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria J.","family":"Monte","sequence":"additional","affiliation":[{"name":"Laboratory of 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