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Despite using prophylactic antibiotics, infections are still seen. Whether the tissue concentration is above the minimal inhibitory concentration (MIC) for the pathogen is unknown. Thus, the concentration of dicloxacillin in muscle and adipose tissue was measured after intravenous administration, in healthy men.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>MIC for dicloxacillin against <jats:styled-content style=\"fixed-case\"><jats:italic>S. aureus<\/jats:italic><\/jats:styled-content> was determined using the broth macrodilution method. A microdialysis (MD) catheter was placed in the subcutaneous tissue of the abdomen and in the lateral vastus muscle of the thigh of six healthy male volunteers. They were given 2\u00a0g dicloxacillin intravenously. Samples from blood and MD fluid were collected. The unbound dicloxacillin was isolated from plasma. Samples were analysed with high performance liquid chromatography (HPLC).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The maximum concentration was reached in muscle tissue after 0.5\u00a0h and in adipose tissue after 0.8\u00a0h. AUC<jats:sub>0\u20136h<\/jats:sub> for the dicloxacillin concentration in adipose tissue was significantly lower when compared to the unbound dicloxacillin concentration in plasma. The dicloxacillin concentration was above the MIC for sensitive <jats:styled-content style=\"fixed-case\"><jats:italic>S. aureus<\/jats:italic><\/jats:styled-content> for a minimum of 2.3\u00a0h and a median of 4.1\u00a0h in muscle tissue and a minimum of 1.8\u00a0h and a median of 3.2\u00a0h in adipose tissue.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The unbound dicloxacillin concentration in adipose and muscle tissue remained above the MIC for sensitive <jats:styled-content style=\"fixed-case\"><jats:italic>S. aureus<\/jats:italic><\/jats:styled-content>, for a period sufficient for many orthopaedic procedures. Whether this is true in patients with compromised circulation remains to be investigated.<\/jats:p><\/jats:sec>","DOI":"10.1111\/bcp.13468","type":"journal-article","created":{"date-parts":[[2017,11,5]],"date-time":"2017-11-05T23:12:39Z","timestamp":1509923559000},"page":"533-541","source":"Crossref","is-referenced-by-count":8,"title":["Microdialysis as a tool to determine the local tissue concentration of dicloxacillin in man"],"prefix":"10.1111","volume":"84","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5505-938X","authenticated-orcid":false,"given":"Kristian Kraft","family":"Hansen","sequence":"first","affiliation":[{"name":"Orthopaedic Research Unit, Department of Orthopaedic Surgery and Traumatology Odense University Hospital  Clausens alle 26, st 5250 Odense SV Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flemming","family":"Nielsen","sequence":"additional","affiliation":[{"name":"Clinical Pharmacology and Pharmacy, Department of Public Health University of Southern Denmark  Odense SV Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4698-4389","authenticated-orcid":false,"given":"Tore Bjerregaard","family":"Stage","sequence":"additional","affiliation":[{"name":"Clinical Pharmacology and Pharmacy, Department of Public Health University of Southern Denmark  Odense SV Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uffe","family":"J\u00f8rgensen","sequence":"additional","affiliation":[{"name":"Orthopaedic Research Unit, Department of Orthopaedic Surgery and Traumatology Odense University Hospital  Clausens alle 26, st 5250 Odense SV Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ole","family":"Skov","sequence":"additional","affiliation":[{"name":"Orthopaedic Research Unit, Department of Orthopaedic Surgery and Traumatology Odense University Hospital  Clausens alle 26, st 5250 Odense SV Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lasse E.","family":"Rasmussen","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery Vejle Hospital  Vejle Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,12,7]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1086\/512192"},{"key":"e_1_2_10_3_1","unstructured":"LundB KlamerF JohannessenT.Proteseinfektion i knogler og led2010. 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