{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T16:14:14Z","timestamp":1791389654707,"version":"4.3.3"},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,10,3]],"date-time":"2022-10-03T00:00:00Z","timestamp":1664755200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Brit J Clinical Pharma"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>Determining dihydropyrimidine dehydrogenase (DPD) activity by measuring patient's uracil (U) plasma concentration is mandatory before fluoropyrimidine (FP) administration in France. In this study, we aimed to refine the pre\u2010analytical recommendations for determining U and dihydrouracil (UH<jats:sub>2<\/jats:sub>) concentrations, as they are essential in reliable DPD\u2010deficiency testing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>U and UH<jats:sub>2<\/jats:sub> concentrations were collected from 14 hospital laboratories. Stability in whole blood and plasma after centrifugation, the type of anticoagulant and long\u2010term plasma storage were evaluated. The variation induced by time and temperature was calculated and compared to an acceptability range of \u00b120%. Inter\u2010occasion variability (IOV) of U and UH<jats:sub>2<\/jats:sub> was assessed in 573 patients double sampled for DPD\u2010deficiency testing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Storage of blood samples before centrifugation at room temperature (RT) should not exceed 1\u00a0h, whereas cold (+4\u00b0C) storage maintains the stability of uracil after 5\u00a0hours. For patients correctly double sampled, IOV of U reached 22.4% for U (SD\u2009=\u200917.9%, range\u2009=\u20090\u201399%). Notably, 17% of them were assigned with a different phenotype (normal or DPD\u2010deficient) based on the analysis of their two samples. For those having at least one non\u2010compliant sample, this percentage increased up to 33.8%. The moment of blood collection did not affect the DPD phenotyping result.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Caution should be taken when interpreting U concentrations if the time before centrifugation exceeds 1\u00a0hour at RT, since it rises significantly afterwards. Not respecting the pre\u2010analytical conditions for DPD phenotyping increases the risk of DPD status misclassification.<\/jats:p><\/jats:sec>","DOI":"10.1111\/bcp.15536","type":"journal-article","created":{"date-parts":[[2022,9,15]],"date-time":"2022-09-15T04:59:38Z","timestamp":1663217978000},"page":"762-772","source":"Crossref","is-referenced-by-count":26,"title":["Quantitative impact of pre\u2010analytical process on plasma uracil when testing for dihydropyrimidine dehydrogenase deficiency"],"prefix":"10.1111","volume":"89","author":[{"given":"Maud","family":"Maillard","sequence":"first","affiliation":[{"name":"Laboratoire de Pharmacologie, Institut Claudius Regaud, IUCT\u2010Oncopole et Centre de Recherches en Canc\u00e9rologie de Toulouse, Inserm UMR1037 Universit\u00e9 Paul Sabatier  Toulouse France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3113-7301","authenticated-orcid":false,"given":"Manon","family":"Launay","sequence":"additional","affiliation":[{"name":"Laboratoire de Pharmacologie et Toxicologie CHU de Saint\u2010Etienne  Saint\u2010Etienne France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1379-858X","authenticated-orcid":false,"given":"Bernard","family":"Royer","sequence":"additional","affiliation":[{"name":"Laboratoire de Pharmacologie Clinique et Toxicologie CHU Besan\u00e7on and Univ. Bourgogne Franche\u2010Comt\u00e9, INSERM, EFS BFC, UMR1098, Interactions H\u00f4te\u2010Greffon\u2010Tumeur\/Ing\u00e9nierie Cellulaire et G\u00e9nique  Besan\u00e7on France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9r\u00f4me","family":"Guitton","sequence":"additional","affiliation":[{"name":"Laboratoire de Pharmacologie Toxicologie CHU de Lyon  Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5846-3847","authenticated-orcid":false,"given":"Elodie","family":"Gautier\u2010Veyret","sequence":"additional","affiliation":[{"name":"Laboratoire de Pharmacologie, Pharmacog\u00e9n\u00e9tique et Toxicologie CHU Grenoble\u2010Alpes et Universit\u00e9 Grenoble\u2010Alpes, laboratoire HP2, INSERM U1300  Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Broutin","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Biologie et Pathologie 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