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Although imatinib mesylate (IM<\/jats:styled-content>) is effective for CML<\/jats:styled-content> treatment, patients in accelerated and blastic phases of the disease are often refractory to this therapy, and there are also cases of IM<\/jats:styled-content> resistance in patients in the chronic phase. Therefore, potential new drugs are being investigated to improve the efficiency of the therapy of CML<\/jats:styled-content> such as snake venoms and their compounds. In this investigation, B<\/jats:styled-content>othrops pirajai<\/jats:italic> L\u2010amino acid oxidase (BpirLAAO<\/jats:styled-content>\u2010I) effect on normal peripheral blood mononuclear cells (PBMC<\/jats:styled-content>) and on BCR<\/jats:styled-content>\u2010ABL<\/jats:styled-content>+<\/jats:sup> cell line was assessed to explore its potential against leukaemic cells. MTT<\/jats:styled-content> viability assay, lymphocyte subsets quantification and cell activation markers expression were performed to evaluate BpirLAAO<\/jats:styled-content>\u2010I effect on normal PBMC<\/jats:styled-content>. The effect of BpirLAAO<\/jats:styled-content>\u2010I on HL<\/jats:styled-content>\u201060 and HL<\/jats:styled-content>\u201060.BCR<\/jats:styled-content>\u2010ABL<\/jats:styled-content> cell lines was assessed by apoptosis detection. BpirLAAO<\/jats:styled-content>\u2010I was able to induce apoptosis in HL<\/jats:styled-content>\u201060 and HL<\/jats:styled-content>\u201060.BCR<\/jats:styled-content>\u2010ABL<\/jats:styled-content> cell lines in a dose\u2010dependent manner, promoted caspases 3, 8 and 9 activation and enhanced IM<\/jats:styled-content> effect while not affecting the viability of normal cells. In addition, BpirLAAO<\/jats:styled-content>\u2010I promoted immune cells activation and lymphocytes subsets changes on normal PBMC<\/jats:styled-content>. The results indicate that BpirLAAO<\/jats:styled-content>\u2010I induces apoptosis and potentiates IM<\/jats:styled-content> effect on BCR<\/jats:styled-content>\u2010ABL<\/jats:styled-content>+<\/jats:sup> cells.<\/jats:p>","DOI":"10.1111\/bcpt.12073","type":"journal-article","created":{"date-parts":[[2013,4,3]],"date-time":"2013-04-03T19:29:20Z","timestamp":1365017360000},"page":"103-112","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["L\u2010Amino Acid Oxidase Isolated from B<\/scp>othrops pirajai<\/i> Induces Apoptosis in BCR<\/scp>\u2010ABL<\/scp>\u2010Positive Cells and Potentiates Imatinib Mesylate Effect"],"prefix":"10.1111","volume":"113","author":[{"given":"Sandra M.","family":"Burin","sequence":"first","affiliation":[{"name":"Department of Clinical, Toxicological and Bromatological Analysis School of Pharmaceutical Sciences of Ribeir\u00e3o Preto University of S\u00e3o Paulo, USP Ribeir\u00e3o Preto SP 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