{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T10:23:53Z","timestamp":1792319033682,"version":"4.4.0"},"reference-count":47,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2013,7,9]],"date-time":"2013-07-09T00:00:00Z","timestamp":1373328000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Basic Clin Pharma Tox"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic><jats:styled-content style=\"fixed-case\">V<\/jats:styled-content>ernonia scorpioides<\/jats:italic> (<jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>am.) <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>ers., popularly known as <jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>nxuga, <jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>rva\u2010de\u2010<jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>\u00e3o <jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>im\u00e3o and <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>irac\u00e1, has been used in folk medicine for its anti\u2010inflammatory, wound healing and antimicrobial properties. Two polyacetylenes, 5\u2010octa\u20102,4,6\u2010triynyl\u2010furan\u20102(5<jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>)\u2010one (<jats:bold>1<\/jats:bold>) and 8\u2032\u2010hydroxy 3\u20104 dihydrovernoniyne (<jats:bold>2<\/jats:bold>), were isolated from the dichloromethane extract fraction of <jats:italic><jats:styled-content style=\"fixed-case\">V<\/jats:styled-content>.\u00a0scorpioides<\/jats:italic>. In this study, polyacetylene <jats:bold>1<\/jats:bold> demonstrated a more potent cytotoxic activity than <jats:bold>2<\/jats:bold> in the tumour cell lines examined, and cytotoxicity was found to be comparable to a commercial drug (<jats:italic>p<\/jats:italic>\u00a0&gt;\u00a00.05) in melanoma cells. No significant cytotoxic effect was observed in normal cell lines. Furthermore, polyacetylene <jats:bold>1<\/jats:bold> induced an <jats:italic>in vitro<\/jats:italic> increase in caspase\u20103 activity in <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>16<jats:styled-content style=\"fixed-case\">F<\/jats:styled-content>10 cells. When polyacetylene <jats:bold>1<\/jats:bold> was administered intraperitoneally (i.p.) in mice, a reduction in solid tumour volume and metastasis was observed in mice injected with <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>16<jats:styled-content style=\"fixed-case\">F<\/jats:styled-content>10 cells. An increase in locomotor activity was also observed in mice with solid tumours, and an inhibition of mechanical hypersensitivity was observed in a mouse model of metastasis. Notably, no significant morphological change was observed in several organs harvested from the treated mice. In conclusion, <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> anticancer activity of polyacetylene <jats:bold>1<\/jats:bold> was consistently observed and involved the induction of apoptosis by the activation of caspase\u20103. The anticancer activity demonstrated by polyacetylene <jats:bold>1<\/jats:bold>, together with the absence of preliminary toxicological effects, represents a new and interesting option for the management of neoplastic disease.<\/jats:p>","DOI":"10.1111\/bcpt.12098","type":"journal-article","created":{"date-parts":[[2013,6,13]],"date-time":"2013-06-13T12:18:11Z","timestamp":1371125891000},"page":"307-315","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Cytotoxic, Antitumour and Antimetastatic Activity of Two New Polyacetylenes Isolated from <i><scp>V<\/scp>ernonia scorpioides<\/i> (<scp>L<\/scp>am.) 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