{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T13:26:08Z","timestamp":1791206768306,"version":"4.1.0"},"reference-count":43,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,2,20]],"date-time":"2015-02-20T00:00:00Z","timestamp":1424390400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Basic Clin Pharma Tox"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Gelatinases play important roles in tumour invasion and metastasis and are thus considered promising targets for cancer therapy. In this study, a new single\u2010chain variable fragment (scFv)\u2010based fusion protein Fv\u2010LDP, composed of the anti\u2010gelatinases scFv and lidamycin apoprotein (LDP), was prepared, and its combination with angiogenesis inhibitor Endostar was then investigated. The fusion protein Fv\u2010LDP specifically bound to various tumour cells, and its binding capability to human pulmonary giant cell carcinoma (PG) cells was higher than that of LDP. Fv\u2010LDP inhibited the expression and secretion of gelatinases and could be internalized into tumour cells via endocytosis. Fv\u2010LDP also suppressed the growth of human hepatoma cells and murine hepatoma 22 transplanted in Kunming mice in various degrees. In addition, Endostar could enhance the synergistic or additive inhibition of Fv\u2010LDP on the growth, migration or invasion of human hepatoma cells shown by a colony formation assay and a transwell\u2010based migration or invasion assay, respectively. <jats:italic>In vivo<\/jats:italic>, Fv\u2010LDP\/Endostar combination showed a significantly synergistic effect on the growth of a human hepatoma xenograft, with an inhibition rate of 80.8% compared with the Fv\u2010LDP (44.1%) or Endostar (8.9%)\u2010treated group. The above\u2010mentioned results indicate that the fusion protein Fv\u2010LDP is effective against transplantable hepatoma in mice and human hepatoma xenografts in athymic mice. Moreover, Endostar can potentiate the inhibition effect of Fv\u2010LDP on the growth of human hepatoma cells and xenografts. These data will provide a new combined strategy for improving the therapeutic efficacy of treatments for hepatoma or other gelatinase\u2010overexpressing tumours.<\/jats:p>","DOI":"10.1111\/bcpt.12379","type":"journal-article","created":{"date-parts":[[2015,1,23]],"date-time":"2015-01-23T08:20:10Z","timestamp":1422001210000},"page":"105-116","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["A Gelatinases\u2010targeting scFv\u2010based Fusion Protein Shows Enhanced Antitumour Activity with Endostar against Hepatoma"],"prefix":"10.1111","volume":"117","author":[{"given":"Ruijuan","family":"Gao","sequence":"first","affiliation":[{"name":"Department of Oncology Institute of Medicinal Biotechnology Chinese Academy of Medical Sciences &amp; Peking Union Medical College Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Oncology Institute of Medicinal Biotechnology Chinese Academy of Medical Sciences &amp; 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