{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T16:51:11Z","timestamp":1772815871427,"version":"3.50.1"},"reference-count":47,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,1,14]],"date-time":"2016-01-14T00:00:00Z","timestamp":1452729600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Counsel of Technological and Scientific Development - CNPq (Brazil)"},{"name":"Research Foundation for the State of Piau\u00ed - FAPEPI","award":["Edital FAPEPI\/SESAPI\/MS\/CNPq\/PPSUS\/No 003\/2013"],"award-info":[{"award-number":["Edital FAPEPI\/SESAPI\/MS\/CNPq\/PPSUS\/No 003\/2013"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Basic Clin Pharma Tox"],"published-print":{"date-parts":[[2016,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Diarrhoea is a significant health problem for children in developing countries that causes more than 1 million deaths annually. This study aimed to evaluate the antidiarrhoeal effect of sulphated polysaccharide (<jats:styled-content style=\"fixed-case\">PLS<\/jats:styled-content>) from the alga <jats:italic>Gracilaria caudata<\/jats:italic> in rodents. For the evaluation, acute diarrhoea was induced in Wistar rats (150\u2013200 g) by administration of castor oil (10 mg\/kg). Then, different parameters, including enteropooling and gastrointestinal transit and its pharmacological modulation by opioid and cholinergic pathways, were assessed using activated charcoal in Swiss Mice (25\u201330 g). Secretory diarrhoea was examined using cholera toxin (CT) (1 mg\/loop)\u2010treated, isolated intestinal loops from Swiss mice (25\u201330 g), which were also used to examine fluid secretion, loss of chloride ions into the intestinal lumen and absorption. In addition, a <jats:styled-content style=\"fixed-case\">GM<\/jats:styled-content>1\u2010dependent <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> was used to evaluate the interaction between <jats:styled-content style=\"fixed-case\">PLS<\/jats:styled-content>, CT and the <jats:styled-content style=\"fixed-case\">GM<\/jats:styled-content>1 receptor. Pre\u2010treatment with <jats:styled-content style=\"fixed-case\">PLS<\/jats:styled-content> (10, 30 and 90 mg\/kg) reduced faecal mass, diarrhoeal faeces and enteropooling. However, 90 mg\/kg more effectively reduced these symptoms; therefore, it was used as the standard dose in subsequent experiments. Gastrointestinal transit was also reduced by <jats:styled-content style=\"fixed-case\">PLS<\/jats:styled-content> treatment via a cholinergic mechanism. Regarding the diarrhoea caused by CT, <jats:styled-content style=\"fixed-case\">PLS<\/jats:styled-content> reduced all study parameters, and the <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> showed that <jats:styled-content style=\"fixed-case\">PLS<\/jats:styled-content> can interact with both the <jats:styled-content style=\"fixed-case\">GM<\/jats:styled-content>1 receptor and CT. These results show that <jats:styled-content style=\"fixed-case\">PLS<\/jats:styled-content> from <jats:italic>G. caudata<\/jats:italic> effectively improved the parameters observed in acute and secretory diarrhoea, which affects millions of people, and may lead to the development of a new alternative therapy for this disease.<\/jats:p>","DOI":"10.1111\/bcpt.12531","type":"journal-article","created":{"date-parts":[[2015,11,19]],"date-time":"2015-11-19T10:20:43Z","timestamp":1447928443000},"page":"440-448","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Sulphated Polysaccharide Isolated from the Seaweed <i>Gracilaria caudata<\/i> Exerts an Antidiarrhoeal Effect in Rodents"],"prefix":"10.1111","volume":"118","author":[{"given":"Douglas S.","family":"Costa","sequence":"first","affiliation":[{"name":"Medicinal Plant Research Center NPPM Postgraduate Program in Pharmacology Federal University of Piau\u00ed Teresina Piau\u00ed Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thiago S. 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