{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T02:21:25Z","timestamp":1769048485006,"version":"3.49.0"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,1,14]],"date-time":"2016-01-14T00:00:00Z","timestamp":1452729600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","award":["SAF2012\u201036271"],"award-info":[{"award-number":["SAF2012\u201036271"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Basic Clin Pharma Tox"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Chemokines are chemotactic cytokines whose involvement in nociceptive processing is being increasingly recognized. Based on the previous description of the involvement of <jats:styled-content style=\"fixed-case\">CC<\/jats:styled-content> chemokine receptor type 1 (<jats:styled-content style=\"fixed-case\">CCR<\/jats:styled-content>1) in pathological pain, we have assessed the participation of <jats:styled-content style=\"fixed-case\">CCR<\/jats:styled-content>1 and its endogenous ligands <jats:styled-content style=\"fixed-case\">CCL<\/jats:styled-content>3 and <jats:styled-content style=\"fixed-case\">CCL<\/jats:styled-content>5 in hyperalgesia and allodynia in mice after acute inflammation with carrageenan and chronic inflammation with complete Freund's adjuvant (<jats:styled-content style=\"fixed-case\">CFA<\/jats:styled-content>). The subcutaneous administration of the <jats:styled-content style=\"fixed-case\">CCR<\/jats:styled-content>1 antagonist J113863 (3\u201330 mg\/kg; 30 min. before) dose dependently inhibited carrageenan\u2010 and <jats:styled-content style=\"fixed-case\">CFA<\/jats:styled-content>\u2010evoked thermal hyperalgesia and mechanical allodynia produced by <jats:styled-content style=\"fixed-case\">CFA<\/jats:styled-content>, but not by carrageenan. The maximal dose of J113863 did not modify the increase in paw thickness induced by carrageenan or <jats:styled-content style=\"fixed-case\">CFA<\/jats:styled-content>. An almost ten times augmentation of <jats:styled-content style=\"fixed-case\">CCL<\/jats:styled-content>3 levels was detected by <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> assays in both carrageenan and <jats:styled-content style=\"fixed-case\">CFA<\/jats:styled-content> paws, but not in spinal cords of inflamed mice, whereas <jats:styled-content style=\"fixed-case\">CCL<\/jats:styled-content>5 concentrations remained unaltered. Accordingly, a marked increase of <jats:styled-content style=\"fixed-case\">CCL<\/jats:styled-content>3 <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression was observed in inflamed paws, with <jats:styled-content style=\"fixed-case\">CCL<\/jats:styled-content>3 protein detected in neutrophils and macrophages by immunohistochemical experiments. The intraplantar administration of an anti\u2010<jats:styled-content style=\"fixed-case\">CCL<\/jats:styled-content>3 antibody (0.3\u20133 \u03bcg) blocked thermal hyperalgesia in carrageenan\u2010 and <jats:styled-content style=\"fixed-case\">CFA<\/jats:styled-content>\u2010inflamed mice as well as <jats:styled-content style=\"fixed-case\">CFA<\/jats:styled-content>\u2010evoked mechanical allodynia. Our data suggest that the increased concentrations of <jats:styled-content style=\"fixed-case\">CCL<\/jats:styled-content>3 present in inflamed tissues can be involved in acute and chronic inflammatory hyperalgesia as well as in chronic mechanical allodynia, and that these hypernociceptive symptoms can be counteracted by its neutralization with an antibody or by the blockade of <jats:styled-content style=\"fixed-case\">CCR<\/jats:styled-content>1 receptors.<\/jats:p>","DOI":"10.1111\/bcpt.12543","type":"journal-article","created":{"date-parts":[[2015,12,12]],"date-time":"2015-12-12T21:48:25Z","timestamp":1449956905000},"page":"32-40","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Involvement of <scp>CC<\/scp> Chemokine Receptor 1 and <scp>CCL<\/scp>3 in Acute and Chronic Inflammatory Pain in Mice"],"prefix":"10.1111","volume":"119","author":[{"given":"Mar\u00eda","family":"Llori\u00e1n\u2010Salvador","sequence":"first","affiliation":[{"name":"Laboratory of Pharmacology School of Medicine University Institute of Oncology of Asturias (IUOPA) University of Oviedo Asturias Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Gonz\u00e1lez\u2010Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmacology School of Medicine University Institute of Oncology of Asturias (IUOPA) University of Oviedo Asturias Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Lastra","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmacology School of Medicine University Institute of Oncology of Asturias (IUOPA) University of Oviedo Asturias Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Teresa","family":"Fern\u00e1ndez\u2010Garc\u00eda","sequence":"additional","affiliation":[{"name":"Molecular Histopathology in Animal Models of Cancer Unit IUOPA University of Oviedo Asturias Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agust\u00edn","family":"Hidalgo","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmacology School of Medicine University Institute of Oncology of Asturias (IUOPA) University of Oviedo Asturias Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis","family":"Men\u00e9ndez","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmacology School of Medicine University Institute of Oncology of Asturias (IUOPA) University of Oviedo Asturias Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Baamonde","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmacology School of Medicine University Institute of Oncology of Asturias (IUOPA) University of Oviedo Asturias Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,1,14]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresrev.2008.12.002"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/ACO.0b013e32830eb69d"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2013.10.004"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.2174\/1871528114666150114170004"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.173.1.594"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0406030102"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.3171\/2010.12.SPINE10480"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.4.2120"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.21-14-05027.2001"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2013.11.055"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pain.2010.02.025"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2010.07.085"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2007.04874.x"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0105883"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1124\/pr.113.007724"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep10278"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/pros.22891"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0115120"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/bcpt.12099"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/bcpt.12265"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2011-201605"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm0004244"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.169.11.6467"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-0270(01)00483-6"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:CEMN.0000018621.58328.ea"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2007.061199"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0706912"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5752-09.2010"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1186\/1744-8069-7-74"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pain.2012.03.008"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/aws330"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0901167"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2011.04548.x"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2013.09.019"}],"container-title":["Basic &amp; Clinical Pharmacology &amp; Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fbcpt.12543","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fbcpt.12543","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bcpt.12543","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T05:15:02Z","timestamp":1696310102000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/bcpt.12543"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,1,14]]},"references-count":35,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2016,7]]}},"alternative-id":["10.1111\/bcpt.12543"],"URL":"https:\/\/doi.org\/10.1111\/bcpt.12543","archive":["Portico"],"relation":{},"ISSN":["1742-7835","1742-7843"],"issn-type":[{"value":"1742-7835","type":"print"},{"value":"1742-7843","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,1,14]]},"assertion":[{"value":"2015-09-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-12-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-01-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}