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Uroplakins are unique urinary transmembrane proteins of urothelium, which may become potential targets of CP<\/jats:styled-content> metabolites and reactive free radicals. Natural compounds, especially those rich in thiols, have shown protective effects against CP<\/jats:styled-content>\u2010induced HC<\/jats:styled-content>. In this study, we studied the modulatory effect of the thiol\u2010rich compound S<\/jats:italic>\u2010allyl cysteine (SAC<\/jats:styled-content>) on the mRNA<\/jats:styled-content> level of uroplakin II<\/jats:styled-content> by real\u2010time polymerase chain reaction and expression of uroplakin II<\/jats:styled-content> protein by immunoblotting. SAC<\/jats:styled-content> (150 mg\/kg) showed significant (p<\/jats:italic> < 0.001) protective effects against CP<\/jats:styled-content> (200 mg\/kg)\u2010induced alteration in mRNA<\/jats:styled-content> level and protein expression of uroplakin II<\/jats:styled-content>. SAC<\/jats:styled-content> also protected animals from CP<\/jats:styled-content>\u2010induced HC<\/jats:styled-content> as assessed by gross morphological examination of urinary bladder. When compared with mercaptoethane sulphonic acid (mesna) (40 mg\/kg), a known thiol\u2010rich drug used in clinical application, SAC was found to be more efficacious in affording protection in urinary bladder tissues. Role of uroplakins in CP<\/jats:styled-content>\u2010induced urinary bladder toxicity has not been well investigated. This study demonstrated that uroplakins may be the potential target of toxic metabolites of CP<\/jats:styled-content> and natural compounds such as SAC<\/jats:styled-content> have the capacity to modulate their expression leading to reduced toxicity burden on the urinary bladder epithelium.<\/jats:p>","DOI":"10.1111\/bcpt.12627","type":"journal-article","created":{"date-parts":[[2016,5,28]],"date-time":"2016-05-28T11:56:42Z","timestamp":1464436602000},"page":"598-603","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Cyclophosphamide\u2010induced Down\u2010Regulation of Uroplakin II<\/scp> in the Mouse Urinary Bladder Epithelium is Prevented by S<\/i>\u2010Allyl Cysteine"],"prefix":"10.1111","volume":"119","author":[{"given":"Sayed Aliul Hasan","family":"Abdi","sequence":"first","affiliation":[{"name":"Department of Medical Elementology & Toxicology Jamia Hamdard (Hamdard University) New Delhi India"},{"name":"Department of Pharmacology Jamia Hamdard (Hamdard University) New Delhi India"},{"name":"Faculty of Interdisciplinary Sciences and Technology Jamia Hamdard, New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abul Kalam","family":"Najmi","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Jamia Hamdard (Hamdard University) New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheikh","family":"Raisuddin","sequence":"additional","affiliation":[{"name":"Department of Medical Elementology & Toxicology Jamia Hamdard (Hamdard University) New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,7,21]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrclinonc.2009.146"},{"key":"e_1_2_9_3_1","first-page":"199","article-title":"Mechanisms of hemorrhagic cystitis","volume":"2","author":"Haldar S","year":"2014","journal-title":"Am J Clin Exp Urol"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1702611"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.4103\/0970-1591.65380"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.12467"},{"key":"e_1_2_9_7_1","doi-asserted-by":"crossref","first-page":"19170","DOI":"10.1016\/S0021-9258(17)30640-3","article-title":"Large scale purification and immunolocalization of bovine uroplakins I, II, and III. 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