{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T11:33:16Z","timestamp":1777721596225,"version":"3.51.4"},"reference-count":39,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,7,12]],"date-time":"2016-07-12T00:00:00Z","timestamp":1468281600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81272274"],"award-info":[{"award-number":["81272274"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Basic Clin Pharma Tox"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Nasopharyngeal carcinoma (<jats:styled-content style=\"fixed-case\">NPC<\/jats:styled-content>) is a refractory tumour, and chemotherapy is one of the primary treatment modalities. Oncoprotein 18 (Op18)\/stathmin is a conserved small cytosolic phosphoprotein and highly expressed in tumours, which plays a vital role in maintaining the malignant phenotype of tumours. Taxol is a clinically widely used chemotherapeutic agent for a broad range of taxol\u2010resistant tumours. This study showed that Op18\/stathmin silencing by <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> interference (<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>i) combined taxol cooperatively improved cellular apoptosis in <jats:styled-content style=\"fixed-case\">CNE<\/jats:styled-content>1 cells mainly via initiating endogenous death receptor pathway, impaired the capabilities of cellular proliferation and cellular migration and down\u2010regulated the half maximal inhibitory concentration (<jats:styled-content style=\"fixed-case\">IC<\/jats:styled-content><jats:sub>50<\/jats:sub>) of taxol, meanwhile decreased the expression of the upstream extracellular regulated kinase 1 (<jats:styled-content style=\"fixed-case\">ERK<\/jats:styled-content>1) <jats:italic>in vitro<\/jats:italic>. Evidence also showed that taxol cytotoxicity was markedly augmented for Op18\/stathmin <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>i in other <jats:styled-content style=\"fixed-case\">NPC<\/jats:styled-content> cells. <jats:italic>In vivo<\/jats:italic> animal experiments have demonstrated that early combination of Op18\/stathmin silencing and taxol evidently inhibited tumourigenicity of <jats:styled-content style=\"fixed-case\">CNE<\/jats:styled-content>1 cells and growth of xenografted tumours in nude mice. Remarkably, silencing Op18\/stathmin by <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>i still promoted transformation of late\u2010stage <jats:styled-content style=\"fixed-case\">CNE<\/jats:styled-content>1 cells in <jats:styled-content style=\"fixed-case\">NPC<\/jats:styled-content>\u2010xenografted tumours from moderately to highly differentiated and inhibited the pleiotropic cytokine interleukin\u201010 (<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201010) autocrine by transplanted tumours. These findings suggest that silencing Op18\/stathmin by <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>i promotes chemosensitization of <jats:styled-content style=\"fixed-case\">NPC<\/jats:styled-content> to taxol and reverses malignant phenotypes of <jats:styled-content style=\"fixed-case\">NPC<\/jats:styled-content>, which provides a new clue for treating drug\u2010resistant tumours.<\/jats:p>","DOI":"10.1111\/bcpt.12633","type":"journal-article","created":{"date-parts":[[2016,6,11]],"date-time":"2016-06-11T13:12:38Z","timestamp":1465650758000},"page":"611-620","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":52,"title":["Silencing Op18\/stathmin by <scp>RNA<\/scp> Interference Promotes the Sensitivity of Nasopharyngeal Carcinoma Cells to Taxol and High\u2010Grade Differentiation of Xenografted Tumours in Nude Mice"],"prefix":"10.1111","volume":"119","author":[{"given":"Xuechi","family":"Lin","sequence":"first","affiliation":[{"name":"Department of Medical Laboratory Changsha Medical University Changsha China"},{"name":"Department of Anatomy, Histology and Embryology Institute of Neuroscience Changsha Medical University Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ting","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Changsha Medical University Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lingxi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Changsha Medical University Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sangyan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Changsha Medical University Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xian","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Changsha Medical University Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Liao","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Changsha Medical University Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Long","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Changsha Medical University Changsha China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fang","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Changsha Medical University Changsha China"},{"name":"Department of Clinical Laboratory the First Affiliated Hospital of Hunan Normal University Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,7,12]]},"reference":[{"key":"e_1_2_10_2_1","first-page":"206","article-title":"Assessment of radiotherapy combined with adjuvant chemotherapy in the treatment of patients with advanced nasopharyngeal carcinoma: a prospective study","volume":"20","author":"Kong F","year":"2015","journal-title":"J BUON"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.5732\/cjc.010.10377"},{"key":"e_1_2_10_4_1","first-page":"326","article-title":"[Medical treatment of nasopharyngeal cancers]","volume":"89","author":"Tebra S","year":"2011","journal-title":"Tunis Med"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncoscience.143"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/cgt.2013.21"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2014.124"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-013-1345-3"},{"key":"e_1_2_10_9_1","first-page":"195","article-title":"Advances in the regulation of the signals on Op18\/stathmin","volume":"11","author":"Lin XC","year":"2007","journal-title":"Life Sci Res"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0109750"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2012.02271.x"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.23767"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-015-0157-2"},{"key":"e_1_2_10_14_1","doi-asserted-by":"crossref","first-page":"1822","DOI":"10.3892\/ol.2015.2954","article-title":"Clinical experience with nanoparticle albumin\u2010bound paclitaxel, a novel taxane anticancer agent, and management of adverse events in females with breast cancer","volume":"9","author":"Takashima S","year":"2015","journal-title":"Oncol Lett"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2013.05.018"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12032-015-0498-x"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/aji.12329"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-015-0331-3"},{"key":"e_1_2_10_19_1","first-page":"265","article-title":"Regulation of the drug resistance of carcinoma cells mediated by Op18\/stathmin","volume":"33","author":"Lin XC","year":"2013","journal-title":"Chem Life"},{"key":"e_1_2_10_20_1","first-page":"54","article-title":"Combination therapy of liposomal paclitaxel and cisplatin as neoadjuvant chemotherapy in locally advanced cervical cancer","volume":"36","author":"Li Y","year":"2015","journal-title":"Eur J Gynaecol Oncol"},{"key":"e_1_2_10_21_1","first-page":"1189","article-title":"Long\u2010term progression\u2010free survival in a metastatic pancreatic cancer patient treated with first\u2010line nab\u2010paclitaxel and gemcitabine","volume":"28","author":"Boeck S","year":"2014","journal-title":"In Vivo"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2011.02.001"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1517\/14728222.2011.620951"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/jgh.12498"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-013-0882-0"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1089\/cbr.2014.1649"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.5483\/BMBRep.2014.47.12.020"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.leukres.2013.11.013"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.201500010"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.3090"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0097512"},{"key":"e_1_2_10_32_1","first-page":"1299","article-title":"Valproic acid overcomes transforming growth factor\u2010beta\u2010mediated sorafenib resistance in hepatocellular carcinoma","volume":"7","author":"Matsuda Y","year":"2014","journal-title":"Int J Clin Exp Pathol"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.558288"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.24230"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/jgh.12594"},{"key":"e_1_2_10_36_1","first-page":"87","article-title":"Evaluation of minichromosome maintenance\u20103 (MCM3) in oral squamous cell carcinoma","volume":"16","author":"Rezvani G","year":"2015","journal-title":"J Dent"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0127568"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2011.5"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00262-011-1019-1"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-10-388538"}],"container-title":["Basic &amp; Clinical Pharmacology &amp; Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fbcpt.12633","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fbcpt.12633","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bcpt.12633","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T12:57:33Z","timestamp":1695733053000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/bcpt.12633"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,7,12]]},"references-count":39,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2016,12]]}},"alternative-id":["10.1111\/bcpt.12633"],"URL":"https:\/\/doi.org\/10.1111\/bcpt.12633","archive":["Portico"],"relation":{},"ISSN":["1742-7835","1742-7843"],"issn-type":[{"value":"1742-7835","type":"print"},{"value":"1742-7843","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,7,12]]},"assertion":[{"value":"2016-03-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-06-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-07-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}