{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T08:09:34Z","timestamp":1791360574540,"version":"4.3.1"},"reference-count":45,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,5,3]],"date-time":"2017-05-03T00:00:00Z","timestamp":1493769600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"FACIAS"},{"name":"Carl Tryggers Foundations"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Basic Clin Pharma Tox"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The impact of euthanasia methods on endocrine and metabolic parameters in rodent tissues and biological fluids is highly relevant for the accuracy and reliability of the data collected. However, few studies concerning this issue are found in the literature. We compared the effects of three euthanasia methods currently used in animal experimentation (i.e. decapitation,\n                    <jats:styled-content style=\"fixed-case\">CO<\/jats:styled-content>\n                    <jats:sub>2<\/jats:sub>\n                    inhalation and pentobarbital injection) on the serum levels of corticosterone, insulin, glucose, triglycerides, cholesterol and a range of free fatty acids in rats. The corticosterone and insulin levels were not significantly affected by the euthanasia protocol used. However, euthanasia by an overdose of pentobarbital (120 mg\/kg intraperitoneal injection) increased the serum levels of glucose, and decreased cholesterol, stearic and arachidonic acids levels compared with euthanasia by\n                    <jats:styled-content style=\"fixed-case\">CO<\/jats:styled-content>\n                    <jats:sub>2<\/jats:sub>\n                    inhalation and decapitation.\n                    <jats:styled-content style=\"fixed-case\">CO<\/jats:styled-content>\n                    <jats:sub>2<\/jats:sub>\n                    inhalation appears to increase the serum levels of triglycerides, while euthanasia by decapitation induced no individual discrepant biomarker level. We conclude that choice of the euthanasia methods is critical for the reliability of serum biomarkers and indicate the importance of selecting adequate euthanasia methods for metabolic analysis in rodents. Decapitation without anaesthesia may be the most adequate method of euthanasia when taking both animal welfare and data quality in consideration.\n                  <\/jats:p>","DOI":"10.1111\/bcpt.12774","type":"journal-article","created":{"date-parts":[[2017,2,28]],"date-time":"2017-02-28T01:02:51Z","timestamp":1488243771000},"page":"113-118","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":96,"title":["The Choice of Euthanasia Method Affects Metabolic Serum Biomarkers"],"prefix":"10.1111","volume":"121","author":[{"given":"Paula","family":"Pierozan","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Biosciences Uppsala University Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fredrik","family":"Jerner\u00e9n","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Biosciences Uppsala University Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusuf","family":"Ransome","sequence":"additional","affiliation":[{"name":"Department of Social and Behavioral Sciences Harvard T.H. Chan School of Public Health Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oskar","family":"Karlsson","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Biosciences Uppsala University Uppsala Sweden"},{"name":"Department of Clinical Neuroscience Center for Molecular Medicine Karolinska Institute Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,5,3]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.0490347"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781444318777"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.10.029"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.applanim.2007.02.004"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplegacy.1957.192.1.79"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1258\/0023677053739747"},{"key":"e_1_2_8_8_1","first-page":"470","article-title":"Carbon dioxide for euthanasia of laboratory animals","volume":"54","author":"Raj ABM","year":"2004","journal-title":"Comp Med"},{"key":"e_1_2_8_9_1","first-page":"695","article-title":"Evaluation of the aesthetics of physical methods of euthanasia of anesthetized rats","volume":"50","author":"Hickman DL","year":"2011","journal-title":"J Am Assoc Lab Anim Sci"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vascn.2014.11.001"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr401232e"},{"key":"e_1_2_8_12_1","volume-title":"American Veterinary Medical Association (AVMA) Guidelines for the Euthanasia of Animals","author":"AVMA","year":"2013"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(92)90451-T"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0091-3057(85)90143-1"},{"key":"e_1_2_8_15_1","first-page":"316","article-title":"Euthanasia by CO(2) inhalation affects potassium levels in mice","volume":"49","author":"Traslavina RP","year":"2010","journal-title":"J Am Assoc Lab Anim Sci"},{"key":"e_1_2_8_16_1","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/S0022-3565(25)30939-0","article-title":"Plasma catecholamine concentrations in unanesthetized rats during sleep, wakefulness, immobilization and after decapitation","volume":"202","author":"Popper CW","year":"1977","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01000294"},{"key":"e_1_2_8_18_1","first-page":"22","article-title":"Serum corticosterone and blood glucose in rats after two jugular vein blood sampling methods: comparison of the stress response","volume":"40","author":"Vachon P","year":"2001","journal-title":"J Am Assoc Lab Anim Sci"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1644\/BHE-003.1"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1249\/00005768-197500710-00003"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI113793"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0026-0495(92)90054-E"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2013.00144"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2335"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.54.12.3458"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrendo.2015.163"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2005-1129"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1177\/0023677215618618"},{"key":"e_1_2_8_29_1","first-page":"1","article-title":"A good death? Report of the Second Newcastle Meeting on Laboratory Animal Euthanasia","volume":"6","author":"Hawkins P","year":"2016","journal-title":"Animals (Basel)"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0162639"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1258\/002367707780378140"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00122.2005"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1080\/07435808409046762"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2826.2008.01711.x"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1983.55.5.1634"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jclinane.2005.03.009"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1213\/00000539-198112000-00007"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1177\/153537020523001012"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000542-198806000-00008"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(87)90126-9"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/ardp.19903230905"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.1982.54"},{"key":"e_1_2_8_43_1","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1016\/S0022-3565(25)33350-1","article-title":"Effects of barbiturates and ethanol on the physical properties of brain membranes","volume":"223","author":"Harris RA","year":"1982","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1080\/01616412.1987.11739789"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(86)91081-8"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0705326"}],"container-title":["Basic &amp; Clinical Pharmacology &amp; Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fbcpt.12774","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bcpt.12774","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,15]],"date-time":"2025-06-15T16:51:24Z","timestamp":1750006284000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/bcpt.12774"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,5,3]]},"references-count":45,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2017,8]]}},"alternative-id":["10.1111\/bcpt.12774"],"URL":"https:\/\/doi.org\/10.1111\/bcpt.12774","archive":["Portico"],"relation":{},"ISSN":["1742-7835","1742-7843"],"issn-type":[{"value":"1742-7835","type":"print"},{"value":"1742-7843","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,5,3]]},"assertion":[{"value":"2016-12-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-02-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-05-03","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}