{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T20:12:02Z","timestamp":1767211922268},"reference-count":34,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,5,3]],"date-time":"2017-05-03T00:00:00Z","timestamp":1493769600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Latvian National Research Programme Biomedicine for Public Health (BIOMEDICINE)"},{"name":"FP7 project InnovaBalt"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Basic Clin Pharma Tox"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Ischaemia in the heart is accompanied by the accumulation of long\u2010chain acylcarnitines (<jats:styled-content style=\"fixed-case\">LCAC<\/jats:styled-content>s) which is one of the multiple factors that contribute to the ischaemia\u2013reperfusion damage development. Long\u2010term pre\u2010treatment that decreases carnitine and <jats:styled-content style=\"fixed-case\">LCAC<\/jats:styled-content> contents also reduces ischaemia\u2013reperfusion (<jats:styled-content style=\"fixed-case\">IR<\/jats:styled-content>) damage; however, the duration of the post\u2010treatment effects is not known. The aim of the study was to assess the post\u2010treatment effects of the carnitine transport (<jats:styled-content style=\"fixed-case\">OCTN<\/jats:styled-content>2) inhibitor, methyl\u2010<jats:styled-content style=\"fixed-case\">GBB<\/jats:styled-content>, on <jats:styled-content style=\"fixed-case\">LCAC<\/jats:styled-content> content and the duration of its cardioprotective effect. Male Wistar rats received methyl\u2010<jats:styled-content style=\"fixed-case\">GBB<\/jats:styled-content> (5 mg\/kg for 28 days), and the anti\u2010infarction effects on Langendorff\u2010perfused hearts and the acylcarnitine profile in cardiac tissues were measured up to 28 days following the end of the treatment. Methyl\u2010<jats:styled-content style=\"fixed-case\">GBB<\/jats:styled-content> pre\u2010treatment for 28 days decreased <jats:styled-content style=\"fixed-case\">LCAC<\/jats:styled-content> heart tissue content by 87%, and the infarct size was decreased by 57%. Fourteen days post\u2010treatment, the <jats:styled-content style=\"fixed-case\">LCAC<\/jats:styled-content> content was still decreased by 69%, and the infarct size was decreased by 32% compared to Control. A significant Pearson correlation (<jats:italic>r<\/jats:italic> = 0.48, <jats:italic>p<\/jats:italic> = 0.026) was found between infarct size and <jats:styled-content style=\"fixed-case\">LCAC<\/jats:styled-content> tissue content in the methyl\u2010<jats:styled-content style=\"fixed-case\">GBB<\/jats:styled-content>\u2010treated rat hearts. The addition of 2 <jats:styled-content style=\"fixed-case\">mM<\/jats:styled-content> carnitine to isolated heart perfusate significantly diminished the methyl\u2010<jats:styled-content style=\"fixed-case\">GBB<\/jats:styled-content>\u2010induced decrease in <jats:styled-content style=\"fixed-case\">LCAC<\/jats:styled-content>s and infarct size. In conclusion, the anti\u2010infarction effect of methyl\u2010<jats:styled-content style=\"fixed-case\">GBB<\/jats:styled-content> continues for at least 2 weeks post\u2010treatment. No less than a 70% decrease in <jats:styled-content style=\"fixed-case\">LCAC<\/jats:styled-content> content is required to protect ischaemic heart tissues, and the decrease in <jats:styled-content style=\"fixed-case\">LCAC<\/jats:styled-content> levels defines the duration of the post\u2010treatment cardioprotective effect of the <jats:styled-content style=\"fixed-case\">OCTN<\/jats:styled-content>2 inhibitor, methyl\u2010<jats:styled-content style=\"fixed-case\">GBB<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/bcpt.12775","type":"journal-article","created":{"date-parts":[[2017,2,28]],"date-time":"2017-02-28T06:03:08Z","timestamp":1488261788000},"page":"106-112","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Decrease in Long\u2010Chain Acylcarnitine Tissue Content Determines the Duration of and Correlates with the Cardioprotective Effect of Methyl\u2010<scp>GBB<\/scp>"],"prefix":"10.1111","volume":"121","author":[{"given":"Janis","family":"Kuka","sequence":"first","affiliation":[{"name":"Laboratory of Pharmaceutical Pharmacology Latvian Institute of Organic Synthesis Riga Latvia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina","family":"Makrecka\u2010Kuka","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmaceutical Pharmacology Latvian Institute of Organic Synthesis Riga Latvia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helena","family":"Cirule","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmaceutical Pharmacology Latvian Institute of Organic Synthesis Riga Latvia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Solveiga","family":"Grinberga","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmaceutical Pharmacology Latvian Institute of Organic Synthesis Riga Latvia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eduards","family":"Sevostjanovs","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmaceutical Pharmacology Latvian Institute of Organic Synthesis Riga Latvia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maija","family":"Dambrova","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmaceutical Pharmacology Latvian Institute of Organic Synthesis Riga Latvia"},{"name":"Faculty of Pharmacy Riga Stradins University Riga Latvia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edgars","family":"Liepinsh","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmaceutical Pharmacology Latvian Institute of Organic Synthesis Riga Latvia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,5,3]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.12475"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi960552n"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)34721-X"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01028.2002"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.14-255901"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2007.10.013"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrendo.2015.129"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.13004"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vph.2015.05.005"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2014.05.002"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1042\/BCJ20160164"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2015.11.014"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2016.01.019"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.1788"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjsports-2015-095906"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0036-1582317"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-7843.2009.00461.x"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2016.05.029"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1124\/dmd.108.023929"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.105.586107"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-7158.2011.01325.x"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1177\/1074248411419502"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF03168229"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-014-2106-3"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2010.00872.x"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2010.00873.x"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vascn.2012.11.001"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00144.2015"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2008.05.447"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2009.09.014"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11897-013-0133-0"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2009.09.024"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00210-013-0849-0"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00395-009-0003-9"}],"container-title":["Basic &amp; Clinical Pharmacology &amp; Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fbcpt.12775","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bcpt.12775","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T16:17:48Z","timestamp":1696349868000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/bcpt.12775"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,5,3]]},"references-count":34,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2017,8]]}},"alternative-id":["10.1111\/bcpt.12775"],"URL":"https:\/\/doi.org\/10.1111\/bcpt.12775","archive":["Portico"],"relation":{},"ISSN":["1742-7835","1742-7843"],"issn-type":[{"value":"1742-7835","type":"print"},{"value":"1742-7843","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,5,3]]},"assertion":[{"value":"2016-11-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-02-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-05-03","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}