{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T15:58:31Z","timestamp":1792166311790,"version":"4.4.0"},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,5,25]],"date-time":"2017-05-25T00:00:00Z","timestamp":1495670400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Basic Clin Pharma Tox"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Nanoscale graphene oxide (<jats:styled-content style=\"fixed-case\">NGO<\/jats:styled-content>) has great potential in biomedicine by the virtue of its facile functionalization and tunable characteristics. Toxicity assessments have therefore become essential prior to its biomedical applications. This study examined the effects of <jats:styled-content style=\"fixed-case\">NGO<\/jats:styled-content> exposure on male reproductive function of adult Wistar rats. Rats were exposed intraperitoneally to three increasing doses, namely low\u2010dose (0.4\u00a0mg\/kg <jats:styled-content style=\"fixed-case\">BW<\/jats:styled-content>), mid\u2010dose (2.0\u00a0mg\/kg <jats:styled-content style=\"fixed-case\">BW<\/jats:styled-content>) and high\u2010dose (10.0\u00a0mg\/kg <jats:styled-content style=\"fixed-case\">BW<\/jats:styled-content>) of <jats:styled-content style=\"fixed-case\">NGO<\/jats:styled-content>. Repeated exposure of <jats:styled-content style=\"fixed-case\">NGO<\/jats:styled-content> for 15 and 30\u00a0days resulted in decreased epididymal sperm counts and elevated sperm abnormalities. Percentage of motile sperms was also significantly reduced due to the exposure. Activities of <jats:styled-content style=\"fixed-case\">SOD<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> GP<\/jats:styled-content>x and malondialdehyde concentration in the testes increased in a dose\u2010specific manner. Results of the study demonstrated that high\u2010dose of <jats:styled-content style=\"fixed-case\">NGO<\/jats:styled-content> resulted in considerable histological damage to testicular tissue which included atrophy of seminiferous tubules with reduction in germinal epithelium, germ cell loss and vacuolization. Low and mid\u2010doses of <jats:styled-content style=\"fixed-case\">NGO<\/jats:styled-content> were not associated with sperm dysfunction or testis damage. Withdrawal of treatment for 30\u00a0days demonstrated significant recovery potential. Histology of epididymis and male fertility potential were not affected due to the <jats:styled-content style=\"fixed-case\">NGO<\/jats:styled-content> exposure. These findings are important for assessment of the risk involved in manufacturing, use and processing of the graphene oxide\u2010based materials towards male reproductive function.<\/jats:p>","DOI":"10.1111\/bcpt.12782","type":"journal-article","created":{"date-parts":[[2017,3,29]],"date-time":"2017-03-29T18:22:37Z","timestamp":1490811757000},"page":"202-210","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["Effects of Nano\u2010Graphene Oxide on Testis, Epididymis and Fertility of Wistar Rats"],"prefix":"10.1111","volume":"121","author":[{"given":"Naresh Kumar","family":"Nirmal","sequence":"first","affiliation":[{"name":"Department of Zoology Centre for Advanced Studies University of Rajasthan Jaipur India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kumud Kant","family":"Awasthi","sequence":"additional","affiliation":[{"name":"National Institute of Animal Welfare MoEF &amp; CC Ballabhgarh 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