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In this context, Toll\u2010like receptors have been demonstrated to play a critical role in the induction of innate and inflammatory responses. Among these, Toll\u2010like receptor 2 (TLR2) is constitutively expressed in tubular epithelial cells (TECs) of the kidney and is also known to mediate ischaemia reperfusion (IR) injury. Adult male C57BL\/6JRj mice were randomized into seven groups (n = 8): a non\u2010operative control group (CTRL) and six interventional groups in which mice were subjected to a 30 min. bilateral renal ischaemia. Immediately before reperfusion, mice were treated either with saline or with TLR2 antibody (clone T2.5) and harvested after ischaemia and reperfusion for 3, 24 and 48 hr. Analysed kidney homogenates of TLR2 antibody\u2010treated mice displayed significantly decreased levels of TLR2 protein after 3 hr of IR compared to saline\u2010treated mice. Accordingly, the degree of AKT phosphorylation was significantly decreased after 3 hr of IR compared to saline\u2010treated animals. TUNEL staining revealed significantly higher apoptosis rates in TLR2 antibody\u2010treated animals compared to saline\u2010treated mice after 3 and 24 hr of IR. Further, a positive correlation between TLR2 protein expression and phosphorylation of AKT as well as a negative correlation with the number of TUNEL\u2010positive cells could be observed. Inhibition of TLR2 and its signalling pathway by a single application of TLR2 antibody results in reduced phosphorylation of AKT and consecutively increased apoptosis.<\/jats:p>","DOI":"10.1111\/bcpt.12896","type":"journal-article","created":{"date-parts":[[2017,8,31]],"date-time":"2017-08-31T11:43:23Z","timestamp":1504179803000},"page":"223-232","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Systemic TLR2 Antibody Application in Renal Ischaemia and Reperfusion Injury Decreases AKT Phosphorylation and Increases Apoptosis in the Mouse Kidney"],"prefix":"10.1111","volume":"122","author":[{"given":"Anja","family":"Urbschat","sequence":"first","affiliation":[{"name":"Department of Biomedicine Aarhus University Aarhus Denmark"},{"name":"Clinic of Urology and Pediatric Urology Medical School Philipps\u2010University Marburg Marburg 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