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Herein, the chelating agent,<jats:italic>N,N<\/jats:italic>\u2019\u2010Bis(2\u2010mercaptoethyl)isophthalamide (<jats:styled-content style=\"fixed-case\">NBMI<\/jats:styled-content>), was examined for its potential ability to entrap<jats:styled-content style=\"fixed-case\">NAPQI<\/jats:styled-content>and p\u2010<jats:styled-content style=\"fixed-case\">BQ<\/jats:styled-content>and to prevent paracetamol\u2010induced liver injury. Both<jats:styled-content style=\"fixed-case\">NBMI<\/jats:styled-content>and the conventional paracetamol antidote<jats:italic>N<\/jats:italic>\u2010acetylcysteine (<jats:styled-content style=\"fixed-case\">NAC<\/jats:styled-content>) were investigated with regard to their abilities to scavenge the<jats:styled-content style=\"fixed-case\">NAPQI<\/jats:styled-content>and p\u2010<jats:styled-content style=\"fixed-case\">BQ<\/jats:styled-content>in a Transient Receptor Potential Ankyrin 1\u2010dependent screening assay. Stoichiometric evaluations indicated that<jats:styled-content style=\"fixed-case\">NBMI<\/jats:styled-content>was able to entrap these metabolites more efficiently than<jats:styled-content style=\"fixed-case\">NAC<\/jats:styled-content>. Furthermore, oral administration of either<jats:styled-content style=\"fixed-case\">NBMI<\/jats:styled-content>(680 mg\/kg) or<jats:styled-content style=\"fixed-case\">NAC<\/jats:styled-content>(680 mg\/kg) prevented the development of the characteristic liver necrosis and elevation of serum alanine aminotransferase in a mouse model for paracetamol\u2010induced liver injury. In summary, these results show that<jats:styled-content style=\"fixed-case\">NBMI<\/jats:styled-content>is able to entrap the toxic metabolites<jats:styled-content style=\"fixed-case\">NAPQI<\/jats:styled-content>and p\u2010<jats:styled-content style=\"fixed-case\">BQ<\/jats:styled-content>and to prevent paracetamol\u2010induced liver injury in mice.<\/jats:p>","DOI":"10.1111\/bcpt.13058","type":"journal-article","created":{"date-parts":[[2018,6,16]],"date-time":"2018-06-16T13:45:10Z","timestamp":1529156710000},"page":"589-593","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["<i>N,N<\/i>\u2019\u2010Bis(2\u2010mercaptoethyl)isophthalamide Binds Electrophilic Paracetamol Metabolites and Prevents Paracetamol\u2010Induced Liver Toxicity"],"prefix":"10.1111","volume":"123","author":[{"given":"Johan L. \u00c5.","family":"Nilsson","sequence":"first","affiliation":[{"name":"Division of Clinical Chemistry and Pharmacology Department of Laboratory Medicine Lund University Lund Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anders","family":"Blomgren","sequence":"additional","affiliation":[{"name":"Division of Clinical Chemistry and Pharmacology Department of Laboratory Medicine Lund University Lund Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulf J.","family":"Nilsson","sequence":"additional","affiliation":[{"name":"Department of Chemistry Centre for Analysis and Synthesis Lund University Lund Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward D.","family":"H\u00f6gest\u00e4tt","sequence":"additional","affiliation":[{"name":"Division of Clinical Chemistry and Pharmacology Department of Laboratory Medicine Lund University Lund Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lars","family":"Grundemar","sequence":"additional","affiliation":[{"name":"Division of Clinical Chemistry and Pharmacology Department of Laboratory Medicine Lund University Lund Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,7,9]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.07-8506com"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/240410a0"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2003-42640"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.20948"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2796.2007.01818.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.5.1327"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/1099-0461(2001)15:1<34::AID-JBT4>3.0.CO;2-U"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0041-008X(82)90017-5"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0272-6386(96)90562-4"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1159\/000136531"},{"key":"e_1_2_7_12_1","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/S0022-3565(25)29663-X","article-title":"Acetaminophen\u2010induced hepatic necrosis. 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