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To assess the sensitivity of the 3T3 cell line, the study compared the changes in mitochondrial membrane potential (<jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>) occurring after exposure to eight chemicals known to demonstrate pro\u2010apoptotic activity (glycerol, isopropanol, ethanol, paracetamol, propranolol, cobalt chloride, formaldehyde and atropine). Five cell lines were used as follows: mouse 3T3 fibroblasts, human epithelial cells (A549, Caco\u20102 and HepG2) and human endothelial cells (<jats:styled-content style=\"fixed-case\">HMEC<\/jats:styled-content>\u20101). Cell sensitivity was assessed based on the total area under and over the dose\u2010response curves (<jats:styled-content style=\"fixed-case\">AUOC<\/jats:styled-content>) in relation to baselines. The 3T3 fibroblasts had the highest <jats:styled-content style=\"fixed-case\">AUOC<\/jats:styled-content> values and were the most sensitive to the action of all the examined chemicals, with the exception of formaldehyde. Significant changes in <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content> between the 3T3 cell line and other cells were observed after cell treatment with atropine (A549, Caco\u20102 or <jats:styled-content style=\"fixed-case\">HMEC<\/jats:styled-content>\u20101 cells vs 3T3 cells, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05), propranolol (A549 vs 3T3 cells, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.01; HepG2 vs 3T3 cells, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05), cobalt chloride (A549 cells vs 3T3 cells, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.01) or ethanol (<jats:styled-content style=\"fixed-case\">HMEC<\/jats:styled-content>\u20101 vs 3T3, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05). Formaldehyde appeared the most toxic compound for Caco\u20102 cells (Caco\u20102 vs 3T3 cells, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05). The surface areas (<jats:styled-content style=\"fixed-case\">AUOC<\/jats:styled-content>) calculated for each other chemical and obtained for HepG2, Caco\u20102, A549 and <jats:styled-content style=\"fixed-case\">HMEC<\/jats:styled-content>\u20101 did not differ significantly between cell lines. We postulate that mouse 3T3 fibroblasts demonstrate significantly higher relative sensitivity to many agents with toxic potential.<\/jats:p>","DOI":"10.1111\/bcpt.13117","type":"journal-article","created":{"date-parts":[[2018,8,23]],"date-time":"2018-08-23T14:13:53Z","timestamp":1535033633000},"page":"199-210","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Differentiated mitochondrial function in mouse 3T3 fibroblasts and human epithelial or endothelial cells in response to chemical exposure"],"prefix":"10.1111","volume":"124","author":[{"given":"Magdalena","family":"Boncler","sequence":"first","affiliation":[{"name":"Department of Haemostasis and Haemostatic Disorders Medical University of Lodz Lodz Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdalena","family":"Lukasiak","sequence":"additional","affiliation":[{"name":"Proteon Pharmaceuticals SA Lodz 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