{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T13:52:45Z","timestamp":1781013165664,"version":"3.54.1"},"reference-count":33,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,10,11]],"date-time":"2018-10-11T00:00:00Z","timestamp":1539216000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005150","name":"Chinese Academy of Medical Sciences","doi-asserted-by":"publisher","award":["CAMS\u20102017\u2010I2M\u20101\u2010011"],"award-info":[{"award-number":["CAMS\u20102017\u2010I2M\u20101\u2010011"]}],"id":[{"id":"10.13039\/501100005150","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Basic Clin Pharma Tox"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Liver fibrosis is a common symptom of non\u2010alcoholic steatohepatitis (<jats:styled-content style=\"fixed-case\">NASH<\/jats:styled-content>) and a worldwide clinical issue. The miR\u2010122\/<jats:styled-content style=\"fixed-case\">HIF<\/jats:styled-content>\u20101\u03b1 signalling pathway is believed to play an important role in the genesis of progressive fibrosis. Isochlorogenic acid B (<jats:styled-content style=\"fixed-case\">ICAB<\/jats:styled-content>), naturally isolated from <jats:italic>Laggera alata<\/jats:italic>, is verified to have antioxidative and hepatoprotective properties. The aim of this study was to investigate the effect of <jats:styled-content style=\"fixed-case\">ICAB<\/jats:styled-content> on liver fibrosis in <jats:styled-content style=\"fixed-case\">NASH<\/jats:styled-content> and its potential protective mechanisms. <jats:styled-content style=\"fixed-case\">NASH<\/jats:styled-content> was induced in a mouse model with a methionine\u2010 and choline\u2010deficient (<jats:styled-content style=\"fixed-case\">MCD<\/jats:styled-content>) diet for 4\u00a0weeks, and <jats:styled-content style=\"fixed-case\">ICAB<\/jats:styled-content> was orally administered every day at three doses (5, 10 and 20\u00a0mg\/kg). Pathological results indicated that <jats:styled-content style=\"fixed-case\">ICAB<\/jats:styled-content> significantly improved the pathological lesions of liver fibrosis. The levels of serum alanine aminotransferase (<jats:styled-content style=\"fixed-case\">ALT<\/jats:styled-content>), aspartate aminotransferase (<jats:styled-content style=\"fixed-case\">AST<\/jats:styled-content>) and hepatic hydroxyproline (Hyp), cholesterol (<jats:styled-content style=\"fixed-case\">CHO<\/jats:styled-content>) and triglyceride (<jats:styled-content style=\"fixed-case\">TG<\/jats:styled-content>) were also significantly decreased by <jats:styled-content style=\"fixed-case\">ICAB<\/jats:styled-content>. In addition, <jats:styled-content style=\"fixed-case\">ICAB<\/jats:styled-content> inhibited hepatic stellate cells (<jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content>s) activation and the expressions of hepatic genes involved in liver fibrosis including <jats:styled-content style=\"fixed-case\">LOX<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> TGF<\/jats:styled-content>\u2010\u03b21, <jats:styled-content style=\"fixed-case\">MCP<\/jats:styled-content>\u20101, <jats:styled-content style=\"fixed-case\">COL<\/jats:styled-content>1\u03b11 and <jats:styled-content style=\"fixed-case\">TIMP<\/jats:styled-content>\u20101. <jats:styled-content style=\"fixed-case\">ICAB<\/jats:styled-content> also attenuated liver oxidative stress through Nrf2 signalling pathway. What is more, the decreased levels of miR\u2010122 and over\u2010expression of hepatic <jats:styled-content style=\"fixed-case\">HIF<\/jats:styled-content>\u20101\u03b1 could be reversed by <jats:styled-content style=\"fixed-case\">ICAB<\/jats:styled-content> treatment. These results simultaneously confirmed that <jats:styled-content style=\"fixed-case\">ICAB<\/jats:styled-content> had a significant protective effect on fibrosis in <jats:styled-content style=\"fixed-case\">NASH<\/jats:styled-content> by inhibiting oxidative stress via Nrf2 and suppressing multiple profibrogenic factors through miR\u2010122\/<jats:styled-content style=\"fixed-case\">HIF<\/jats:styled-content>\u20101\u03b1 signalling pathway.<\/jats:p>","DOI":"10.1111\/bcpt.13122","type":"journal-article","created":{"date-parts":[[2018,9,4]],"date-time":"2018-09-04T16:18:05Z","timestamp":1536077885000},"page":"144-153","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":53,"title":["Protective effect of isochlorogenic acid B on liver fibrosis in non\u2010alcoholic steatohepatitis of mice"],"prefix":"10.1111","volume":"124","author":[{"given":"Xin","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Pharmacy Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing China"},{"name":"State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Huang","sequence":"additional","affiliation":[{"name":"Drug Clinical Trial Institution Wuxi People\u2019 Hospital Nanjing Medical University Wuxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziran","family":"Niu","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Mei","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,10,11]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2015.07.036"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2012.04.001"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dld.2016.02.014"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2014.11.034"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2015.02.041"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1159\/000282081"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms17111795"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/1751-2980.12408"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2014.10.004"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/liv.12633"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2017.09.022"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI63455"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2007.060887"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10585-010-9360-x"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.28889"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0168572"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2007.01.011"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0278-6915(02)00329-0"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1089\/jmf.2009.1204"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep12931"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.5b00398"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1089\/jmf.2005.8.348"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.3797\/scipharm.1108-16"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf303771s"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2017.00929"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2014-306842"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1530\/JOE-14-0662"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.20183"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2008.02.006"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/biof.1203"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2015.12.024"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v21.i13.3893"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.2009.01007.x"}],"container-title":["Basic &amp; Clinical Pharmacology &amp; Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fbcpt.13122","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bcpt.13122","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,14]],"date-time":"2023-09-14T18:36:08Z","timestamp":1694716568000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/bcpt.13122"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,10,11]]},"references-count":33,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2019,2]]}},"alternative-id":["10.1111\/bcpt.13122"],"URL":"https:\/\/doi.org\/10.1111\/bcpt.13122","archive":["Portico"],"relation":{},"ISSN":["1742-7835","1742-7843"],"issn-type":[{"value":"1742-7835","type":"print"},{"value":"1742-7843","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,10,11]]},"assertion":[{"value":"2018-05-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-08-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-10-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}