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In view of the great potential for the development of a new Ph\u03b11\u03b2\u2010based drug, a Ph\u03b11\u03b2 recombinant form (CTK 01512\u20102) has been studied for efficacy and safety. The aim of this study was to evaluate cytotoxic, genotoxic and mutagenic effects of a Ph\u03b11\u03b2 recombinant form and compare it with native Ph\u03b11\u03b2 and \u03c9\u2010conotoxin MVIIA. Cytotoxicity was evaluated using the MTT (3\u2010[4,5\u2010dimethylthiazol\u20102\u2010yl]\u20102,5\u2010diphenyltetrazolium bromide) colourimetric assay in L929 mouse fibroblast cells (0.5\u201010.0\u00a0\u03bcmol\/L). Genotoxic and mutagenic activities were analysed using the alkaline comet assay in peripheral blood and spinal cord, and the micronucleus test in bone marrow from Wistar rats treated by intrathecal injection of CTK 01512\u20102 (200, 500 and 1000\u00a0pmol\/site), native Ph\u03b11\u03b2 (500\u00a0pmol\/site) and \u03c9\u2010conotoxin MVIIA (200\u00a0pmol\/site). CTK 01512\u20102 decreased the cell viability of the L929, showing IC<jats:sub>50<\/jats:sub> of 3.3\u00a0\u00b1\u00a00.1\u00a0\u00b5mol\/L, while the Ph\u03b11\u03b2 and \u03c9\u2010conotoxin MVIIA did not show cytotoxicity (IC<jats:sub>50<\/jats:sub>\u00a0&gt;\u00a05.0\u00a0\u00b5mol\/L). Native and recombinant Ph\u03b11\u03b2 forms induced DNA damage in the spinal cord, but not in peripheral blood. CTK 01512\u20102 at 1000\u00a0pmol\/site increased the micronucleus frequency suggesting mutagenic effects. In conclusion, the recombinant form has cytotoxic, genotoxic and mutagenic effects, evidenced in doses five times above the therapeutic dose.<\/jats:p>","DOI":"10.1111\/bcpt.13171","type":"journal-article","created":{"date-parts":[[2018,11,18]],"date-time":"2018-11-18T06:04:01Z","timestamp":1542521041000},"page":"615-620","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Evaluation of DNA damage in spinal cord and mutagenic effect of a Ph\u03b11\u03b2 recombinant toxin with analgesic properties from the <i>Phoneutria nigriventer<\/i> spider"],"prefix":"10.1111","volume":"124","author":[{"given":"Alessandra Hubner","family":"de Souza","sequence":"first","affiliation":[{"name":"Laboratory of Pharmacology and Toxicology Lutheran University of Brazil (ULBRA) Canoas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luiza Gabriela","family":"da Rosa","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmacology and Toxicology Lutheran University of Brazil (ULBRA) Canoas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michel Rossi","family":"Uliano","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmacology and Toxicology Lutheran University of Brazil (ULBRA) Canoas Brazil"},{"name":"Laboratory of Toxicological Genetics Lutheran University of Brazil (ULBRA) Canoas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lismare","family":"da Silva Prado","sequence":"additional","affiliation":[{"name":"Laboratory of Toxicological Genetics Lutheran University of Brazil (ULBRA) Canoas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alice Gomes","family":"Ferraz","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmacology and Toxicology Lutheran University of Brazil (ULBRA) Canoas Brazil"},{"name":"Laboratory of Toxicological Genetics Lutheran University of Brazil (ULBRA) Canoas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucas Umpierre","family":"Conter","sequence":"additional","affiliation":[{"name":"Laboratory of Cancer Biology Lutheran University of Brazil (ULBRA) Canoas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivana","family":"Grivicich","sequence":"additional","affiliation":[{"name":"Laboratory of Cancer Biology Lutheran University of Brazil (ULBRA) Canoas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eliane","family":"Dallegrave","sequence":"additional","affiliation":[{"name":"Department of Pharmacosciences. 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