{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T21:03:25Z","timestamp":1780520605173,"version":"3.54.1"},"reference-count":54,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,4,2]],"date-time":"2019-04-02T00:00:00Z","timestamp":1554163200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Basic Clin Pharma Tox"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Reactive oxygen species (ROS) is regarded as a critical denominator in nanoparticle toxicology and inflammation. Previously, we have shown that silica nanoparticles sized 50\u00a0nm (Si50) induce release of CXCL8 and IL\u20106 from BEAS\u20102B cells, via mechanisms involving NF\u03baB, p38 MAP kinase and TGF\u2010\u03b1\u2010activated EGF receptor. In the present study, the role of ROS\u2010mediated mechanisms in the concentration\u2010dependent Si50 induction of CXCL8 and IL\u20106 responses was examined. Si50 (200\u00a0\u00b5g\/mL) induced a time\u2010dependent ROS formation and a postponed increase in expression of haem oxygenase (HO\u20101) mRNA and protein. Pre\u2010treatment with the ROS inhibitors N\u2010acetyl cysteine (NAC) and diphenyleneiodonium (DPI) partially attenuated CXCL8 and IL\u20106 responses to 200\u00a0\u00b5g\/mL, but not to 100\u00a0\u00b5g\/mL Si50. The release of TGF\u2010\u03b1 induced by Si50 (200\u00a0\u00b5g\/mL) was significantly reduced by NAC, but not by DPI nor siRNA against NADPH oxidase DUOX\u20101 (siDUOX\u20101). Furthermore, siDUOX\u20101 reduced Si50\u2010induced CXCL8, but not IL\u20106. Both p38 and p65 phosphorylations were inhibited by siDUOX\u20101, but for NAC only p65 phosphorylation reached a significant reduction. Neither NAC nor DPI reduced Si50\u2010induced CXCL8 and IL\u20106 gene expressions. In conclusion, Si50\u2010induced CXCL8 and IL\u20106 involved both ROS\u2010dependent and ROS\u2010independent mechanisms. Notably, the role of ROS seemed restricted to effects of higher concentrations of Si50 and not mediated via the gene expression.<\/jats:p>","DOI":"10.1111\/bcpt.13221","type":"journal-article","created":{"date-parts":[[2019,3,12]],"date-time":"2019-03-12T20:55:43Z","timestamp":1552424143000},"page":"304-314","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Concentration\u2010dependent cytokine responses of silica nanoparticles and role of ROS in human lung epithelial cells"],"prefix":"10.1111","volume":"125","author":[{"given":"Magne","family":"Refsnes","sequence":"first","affiliation":[{"name":"Department of Air Pollution and Noise Norwegian Institute of Public Health Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tonje","family":"Skuland","sequence":"additional","affiliation":[{"name":"Department of Air Pollution and Noise Norwegian Institute of Public Health Oslo 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