{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T05:54:26Z","timestamp":1761630866552},"reference-count":19,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,6,26]],"date-time":"2019-06-26T00:00:00Z","timestamp":1561507200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Basic Clin Pharma Tox"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Urine tests are the commonly accepted methods to control abstinence and adherence to treatment of patients who undergo methadone maintenance treatment (MMT). Depending on various national guidelines and accessibility of techniques, only selected psychoactive substances are routinely tested in urine of MMT patients. In general, they belong to the few groups of compounds: THC, cocaine, amphetamines, opiates, PCP and benzodiazepines. It is, however, well known that patients enrolled in such replacement programmes take psychoactive substances that are not routinely detected by the toxicology laboratories, to escape unexpected tests. Here, we report semiquantitative detection of legal highs taken by the MMT patient, using high\u2010pressure liquid chromatography coupled to the flowing atmospheric pressure afterglow ion source (LC\u2010FAPA\u2010MS). To demonstrate effectivity of this technique, the data were confirmed by quantitative analysis using LC\u2010ESI\u2010MS\/MS. In the analysed sample of MMT patient, a mixture of psychoactive compounds was found, namely 3\u2010MMC (3\u2010methylmethcathinone), pentedrone and methcathinone and determined at the concentrations of 670; 50 and 0.2\u00a0\u00b5g\/mL, respectively. Such fast analytical technique may be useful for the efficient control of substances taken intentionally by MMT patients.<\/jats:p>","DOI":"10.1111\/bcpt.13270","type":"journal-article","created":{"date-parts":[[2019,6,12]],"date-time":"2019-06-12T12:59:11Z","timestamp":1560344351000},"page":"253-258","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Detection of legal highs in the urine of methadone\u2010treated patient by LC\u2010MS"],"prefix":"10.1111","volume":"125","author":[{"given":"Krzysztof","family":"Labuz","sequence":"first","affiliation":[{"name":"Nordhemskliniken, Unit of Addiction Sahlgrenska University Hospital G\u00f6teborg Sweden"},{"name":"The Rydygier Hospital in Krakow, Addiction Outpatient Clinic Krakow Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Piotr","family":"Adamowicz","sequence":"additional","affiliation":[{"name":"Institute of Forensic Research Krak\u00f3w Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Ka\u0142a","sequence":"additional","affiliation":[{"name":"Cracow School of Health Promotion Krak\u00f3w Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Krzysztof","family":"Pyrc","sequence":"additional","affiliation":[{"name":"Microbiology Department, Faculty of Biochemistry, Biophysics and Biotechnology Jagiellonian University Krakow Poland"},{"name":"Virogenetics Laboratory of Virology Malopolska Centre of Biotechnology, Jagiellonian University Krakow Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward","family":"Reszke","sequence":"additional","affiliation":[{"name":"ERTEC\u2010Poland Wroc\u0142aw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Przemyslaw","family":"Mielczarek","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Neurobiology, Faculty of Materials Science and Ceramics AGH University of Science and Technology Krak\u00f3w Poland"},{"name":"Laboratory of Proteomics and Mass Spectrometry, Institute of Pharmacology Polish Academy of Sciences Krakow Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jerzy","family":"Silberring","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Neurobiology, Faculty of Materials Science and Ceramics AGH University of Science and Technology Krak\u00f3w Poland"},{"name":"Centre of Polymer and Carbon Materials Polish Academy of Sciences Zabrze Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marek","family":"Smoluch","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Neurobiology, Faculty of Materials Science and Ceramics AGH University of Science and Technology Krak\u00f3w Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,6,26]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jms.3142"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.3109\/15563650.2011.590812"},{"key":"e_1_2_6_4_1","doi-asserted-by":"crossref","first-page":"4.43.1\u20104.43.10","DOI":"10.1002\/cptx.33","article-title":"Gas chromatography mass spectrometry (GC\u2010MS) for identification of designer stimulants including 2C amines, NBOMe compounds, and cathinones in urine","volume":"74","author":"Ketha H","year":"2017","journal-title":"Curr Protoc Toxicol"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/bku021"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/25.7.525"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2015.04.002"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/bkw076"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.3109\/15563650.2014.885982"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11419-018-0432-y"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200500934"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-78707-7_4"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408347.2018.1439724"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.6262"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2015.07.094"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/bcpt.13059"},{"key":"e_1_2_6_17_1","volume-title":"Molecular Cloning: A Laboratory Manual","author":"Green MR","year":"2012"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1039\/C3AN02067C"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2015.02.049"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac800210s"}],"container-title":["Basic &amp; 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