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Augmentation of this pathway by dietary nitrate has proven favourable effects in several cardiovascular disease models. However, less is known regarding its potential value in pulmonary arterial hypertension (PAH). The aim of this study was to assess the effects of oral inorganic nitrate administration in monocrotaline (MCT)\u2010induced PAH. Male 12\u2010week\u2010old Wistar rats were injected subcutaneously with monocrotaline (MCT, 60\u00a0mg\/kg). Nitrate treatment (0.3 or 1\u00a0mmol\/kg\/d; drinking water) commenced on day 12 following the MCT injection and continued for 16\u00a0days. Nitrate administration did not attenuate right ventricular (RV) hypertrophy, increased lung weight and up\u2010regulated mRNA expression of brain natriuretic peptide. Plasma nitrate and nitrite levels were significantly increased as well as lung nitrate level, whereas nitrite lung level was decreased following nitrate treatment (1\u00a0mmol\/kg\/d). MCT\u2010induced PAH resulted in an increased MnSOD protein level, which was not observed following nitrate treatment. MCT\u2010associated up\u2010regulation of nNOS in the lung appeared to be dose\u2010dependently prevented by nitrate treatment. Western blot analysis did not reveal any differences in eNOS, iNOS, XO or gp91phox expression in the lungs among the groups. In conclusion, nitrate treatment did not significantly attenuate pathological RV and lung remodelling in the rat MCT model of PAH. The suppression of MnSOD and nNOS expression by nitrate could be interpreted as reduced demand of endogenous antioxidant defence in this model.<\/jats:p>","DOI":"10.1111\/bcpt.13309","type":"journal-article","created":{"date-parts":[[2019,8,20]],"date-time":"2019-08-20T08:49:31Z","timestamp":1566290971000},"page":"99-109","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Effects of inorganic nitrate in a rat model of monocrotaline\u2010induced pulmonary arterial hypertension"],"prefix":"10.1111","volume":"126","author":[{"given":"Eva","family":"Malikova","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Toxicology Faculty of Pharmacy Comenius University in Bratislava Bratislava Slovak Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mattias","family":"Carlstr\u00f6m","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pharmacology Karolinska 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