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Unilateral chronic ocular hypertension model of rats was created by the intracameral injection of 3\u00a0\u03bcL Healaflow. The IOP of subjects was monitored. Dynamic morphological changes were evaluated by fundus imaging, OCT and histological examination. Visual function changes were measured by electroretinography and flash visual\u2010evoked potentials. 24 and 72\u00a0hours after injection, the retinal tissue was collected for transcriptome analysis. The expression levels of related genes and proteins were further evaluated by qRT\u2010PCR and Western blotting. The IOP peaked within 1\u00a0day after a single intracameral injection of Healaflow and then decreased gradually within 4\u00a0weeks. Furthermore, the persistently degenerating retinal ganglion cells occurred within 4\u00a0weeks. The visual function of these rats was also impaired. The results of transcriptome analyses, qRT\u2010PCR and Western blotting showed that the expression levels of B2m, Ikzf1 and Stat3 were up\u2010regulated, while the expression levels of Six3 and Prss56 were down\u2010regulated in the retinal tissues. Intracameral injection of Healaflow is an effective approach to induce glaucomatous neurodegeneration in rats. 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