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Haematoxylin\u2010eosin (HE) staining and Masson's trichrome staining were used to evaluate pathological changes. Western blotting was performed to observe expression levels of sirtuin (SIRT) 1, insulin\u2010like growth factor binding protein\u2010related protein 1 (IGFBPrP1) and TGF \u03b21. The activity of SIRT 1 also was detected. Results showed that cucurbitacin B could effectively improve the abnormal liver function, inhibit liver fibrosis and suppress releases of inflammatory factors in mice induced by concanavalin A. Furthermore, cucurbitacin B could down\u2010regulate the expressions of TGF \u03b21 and IGFBPrP1, increase the expression and activity of SIRT 1. Interestingly, when SIRT1 activity was inhibited by EX 527, a selective inhibitor of SIRT 1, the preventive effect of cucurbitacin B was significantly attenuated. Taken together, the above results showed that cucurbitacin B could significantly suppress releases of inflammatory cytokines and improve liver fibrosis induced by concanavalin A in mice, and those may be achieved through SIRT1\/IGFBPrP1\/TGF \u03b21 axis.<\/jats:p>","DOI":"10.1111\/bcpt.13446","type":"journal-article","created":{"date-parts":[[2020,5,26]],"date-time":"2020-05-26T06:44:40Z","timestamp":1590475480000},"page":"371-379","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["SIRT1\/IGFBPrP1\/TGF \u03b21 axis involved in cucurbitacin B ameliorating concanavalin A\u2010induced mice liver fibrosis"],"prefix":"10.1111","volume":"127","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6741-4070","authenticated-orcid":false,"given":"Li","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Pharmacy The Ninth Hospital of Nanchang Nanchang 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