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Cell viability and LDH levels were examined in RAW264.7 cells and H9 cells. RAW264.7 cells were stimulated with <jats:italic>S typhimurium<\/jats:italic> in the presence or absence of Baicalin, and the levels of pro\u2010inflammatory cytokines were detected by enzyme\u2010linked immunosorbent assay (ELISA). The changes in reactive oxygen species (ROS) production were determined by fluorescence microscopy and ELISA. The autophagy and TLR4\/MAPK\/NF\u2010\u03baB signalling pathway were examined by immunofluorescence microscopy, quantitative reverse transcription\u2010polymerase chain reaction and Western blotting. The results indicated that MOI of 30 and duration of autophagy evident at 5\u00a0h were applicable to this study. Baicalin prevented death of macrophages, promoted bactericidal activity, decreased the levels of pro\u2010inflammatory cytokines and ROS and reduced the changes of key biomarkers in autophagy and TLR4\/MAPK\/NF\u2010\u03baB signalling pathway infected by <jats:italic>S typhimurium<\/jats:italic>. TLR4\u2010overexpressed cells, autophagy and TLR4\/MAPK\/NF\u2010\u03baB signalling pathway were activated by <jats:italic>S typhimurium,<\/jats:italic> which was suppressed by Baicalin. Our findings indicated that Baicalin exerts anti\u2010inflammatory and cell\u2010protective effects, and it mediates autophagy by down\u2010regulating the activity of TLR4 infected by <jats:italic>S typhimurium<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/bcpt.13497","type":"journal-article","created":{"date-parts":[[2020,9,21]],"date-time":"2020-09-21T19:08:05Z","timestamp":1600715285000},"page":"241-255","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Baicalin inhibits <i>Salmonella typhimurium<\/i>\u2010induced inflammation and mediates autophagy through TLR4\/MAPK\/NF\u2010\u03baB signalling pathway"],"prefix":"10.1111","volume":"128","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8974-1902","authenticated-orcid":false,"given":"Ling","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Basic Medical Sciences Jinzhou Medical University Jinzhou 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