{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:43:59Z","timestamp":1747183439188,"version":"3.40.5"},"reference-count":18,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,11,15]],"date-time":"2020-11-15T00:00:00Z","timestamp":1605398400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2014\/09861\u20101","2019\/21304\u20104"],"award-info":[{"award-number":["2014\/09861\u20101","2019\/21304\u20104"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Basic Clin Pharma Tox"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Long QT syndrome type 3 (LQT\u20103) is a disease related to abnormal cardiac sodium channel function (Nav 1.5), usually due to augmented late sodium current (I<jats:sub>NaL<\/jats:sub>), and may lead to ventricular fibrillation. Amiodarone is approved for ventricular fibrillation. Thus, we investigated whether pacing frequency impacts the ability of amiodarone to reverse the arrhythmic phenotype observed in LQT\u20103. Anemone neurotoxin 2 (ATX\u2010II, here named only ATX) was used to enhance I<jats:sub>NaL<\/jats:sub> in mice left ventricular myocytes (LVM). A video detector system monitored sarcomere shortening. At 1\u00a0Hz, amiodarone attenuated sarcomere shortening only at 10\u00a0\u00b5mol\/L; at 3 and 5\u00a0Hz, 0.1 and 1\u00a0\u00b5mol\/L amiodarone also reduced sarcomere shortening. However, no effect of amiodarone was observed on time to 50% of sarcomere contraction and relaxation. In LVM exposed to ATX (10\u00a0nmol\/L), an arrhythmic phenotype was observed, and it was more severe when cells were paced at 1\u00a0Hz. Amiodarone failed to reverse the ATX induced phenotype at different pacing frequencies. Thus, our results suggest that amiodarone's ability to reverse arrhythmias induced by augmentation of I<jats:sub>NaL<\/jats:sub> is limited. These findings suggest further experimentation will be required to clarify whether a clinical effect can be ascribed to an effect of amiodarone on other ion channels in LQT\u20103.<\/jats:p>","DOI":"10.1111\/bcpt.13527","type":"journal-article","created":{"date-parts":[[2020,10,30]],"date-time":"2020-10-30T08:35:19Z","timestamp":1604046919000},"page":"615-620","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Impact of pacing frequency in amiodarone interaction with cardiomyocytes near physiological temperature in health and disease conditions"],"prefix":"10.1111","volume":"128","author":[{"given":"Samuel","family":"S. Beserra","sequence":"first","affiliation":[{"name":"Laboratory of CardioBiology Department of Biophysics Paulista School of Medicina Federal University of Sao Paulo  Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2690-912X","authenticated-orcid":false,"given":"Danilo","family":"Roman\u2010Campos","sequence":"additional","affiliation":[{"name":"Laboratory of CardioBiology Department of Biophysics Paulista School of Medicina Federal University of Sao Paulo  Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,11,15]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/openhrt-2019-001195"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1010663"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCEP.114.002321"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ipej.2017.10.011"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-017-2079-7"},{"issue":"23","key":"e_1_2_7_7_1","doi-asserted-by":"crossref","first-page":"e740","DOI":"10.1161\/CIR.0000000000000613","article-title":"2018 american heart association focused update on advanced cardiovascular life support use of antiarrhythmic drugs during and immediately after cardiac arrest: an update to the american heart association guidelines for cardiopulmonary resuscitation and emergency cardiovascular care","volume":"138","author":"Panchal AR","year":"2018","journal-title":"Circulation."},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2020.117814"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.2001.1355"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-22033-1"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vascn.2019.106605"},{"issue":"4","key":"e_1_2_7_12_1","first-page":"732","article-title":"Inotropic and Antiarrhythmic Transmural Actions of Ranolazine in a Cellular Model of Type 3 Long QT Syndrome","volume":"114","author":"Miranda VM","year":"2020","journal-title":"Arq Bras Cardiol."},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/bcpt.13059"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2010.10.011"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1008379"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/phy2.158"},{"issue":"5","key":"e_1_2_7_17_1","first-page":"H1772","article-title":"Temperature and relative contributions of Ca transport systems in cardiac myocyte relaxation","volume":"270","author":"Puglisi JL","year":"1996","journal-title":"Am J Physiol"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep13287"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01085.2005"}],"container-title":["Basic &amp; Clinical Pharmacology &amp; Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bcpt.13527","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/bcpt.13527","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/bcpt.13527","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T09:23:04Z","timestamp":1693560184000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/bcpt.13527"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11,15]]},"references-count":18,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2021,4]]}},"alternative-id":["10.1111\/bcpt.13527"],"URL":"https:\/\/doi.org\/10.1111\/bcpt.13527","archive":["Portico"],"relation":{},"ISSN":["1742-7835","1742-7843"],"issn-type":[{"type":"print","value":"1742-7835"},{"type":"electronic","value":"1742-7843"}],"subject":[],"published":{"date-parts":[[2020,11,15]]},"assertion":[{"value":"2020-06-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-10-26","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-11-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}