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This study evaluated major pathways of DRL\u2010induced relaxation in different artery segments and the possible molecular interactions involved. Rat thoracic aorta, coronary and mesenteric resistance arteries were tested \u201cin vitro\u201d with concentration\u2010response curves to DRL (0.01\u2010100\u00a0\u00b5g\/mL). L\u2010NAME, indomethacin and high KCl were used to evaluate nitric oxide, cyclooxygenase and hyperpolarization\u2010dependent effects. DRL promoted relaxation of all vessels throughout different mechanisms. L\u2010NAME blunted DRL\u2010induced effects only in the aorta and mesenteric resistance artery. By the use of depolarizing KCl solution, vasodilation was reduced in all arteries, while incubation with indomethacin indicated a role of cyclooxygenase\u2010derived factors for DRL effects in mesenteric and coronary arteries, but not in the aorta. Molecular docking results suggested interactions between DRL and heparan sulphate, CD31 and other glycans present on the membrane surface. These data indicate that the mechanisms involved in DRL\u2010mediated vasodilation vary between conductance and resistance arteries of different origins, and these effects may be related to the capacity of DRL to bind a diversity of glycans, especially heparan sulphate, a proposed mechanoreceptor for nitric oxide synthase and cyclooxygenase activation.<\/jats:p>","DOI":"10.1111\/bcpt.13614","type":"journal-article","created":{"date-parts":[[2021,5,16]],"date-time":"2021-05-16T07:23:35Z","timestamp":1621149815000},"page":"130-138","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Differential vasodilator effect of <i>Dioclea rostrata<\/i> lectin in conductance and resistance arteries: Mechanisms and glycoconjugate binding relationships"],"prefix":"10.1111","volume":"129","author":[{"given":"Tatiani","family":"Botelho","sequence":"first","affiliation":[{"name":"Department of Physiological Sciences Federal University of Espirito Santo  Vitoria Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5730-8829","authenticated-orcid":false,"given":"Vinicius J.S.","family":"Osterne","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Federal University of Ceara  Fortaleza Brazil"},{"name":"Department of Nutrition State University of Ceara  Fortaleza Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9279-5298","authenticated-orcid":false,"given":"Vanir R.","family":"Pinto\u2010Junior","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Federal University of Ceara  Fortaleza Brazil"},{"name":"Department of Physics Federal University of Ceara  Fortaleza Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Messias V.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Federal University of Ceara  Fortaleza Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5791-6170","authenticated-orcid":false,"given":"Benildo S.","family":"Cavada","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Federal University of Ceara  Fortaleza Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2220-4552","authenticated-orcid":false,"given":"Kyria S.","family":"Nascimento","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Federal University of Ceara  Fortaleza Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4340-6364","authenticated-orcid":false,"given":"Leonardo","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Department of Physiological Sciences Federal University of Espirito Santo  Vitoria Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,5,30]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwh122"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2011.03.010"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18061242"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18061164"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2008.09.042"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(98)00021-0"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2020.06.161"},{"issue":"2","key":"e_1_2_7_9_1","first-page":"770","article-title":"Antifungal activity of lectins against yeast of vaginal secretion. 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