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Therefore, this study aimed to investigate the effects of dipyrone and ASA on the contractions of distal cauda epididymis duct, serum testosterone levels and sperm parameters in rats. Firstly, we checked the in vitro effects of dipyrone and ASA (10\u20131000\u2009\u03bcM) on the contractions of distal cauda epididymis duct by pharmacological experiments. We also evaluated the effects of in vivo treatment with dipyrone and ASA 100\u2009mg\/kg (p.o.) for 15\u2009days on epididymal duct contractions, serum testosterone levels and sperm parameters. In vitro dipyrone or ASA decreased the epididymal duct contractions induced by phenylephrine or carbachol. We observed that in vivo treatment with both drugs decreased the daily sperm production, serum testosterone levels and sperm count through epididymis without altering the epididymal duct contractions and sperm transit time through epididymis. In conclusion, in vitro dipyrone and ASA were able to diminish the contractions of epididymal duct, whilst in vivo administration decreased the sperm count throughout epididymis as a consequence of a low sperm production caused by reduced testosterone levels.<\/jats:p>","DOI":"10.1111\/bcpt.13633","type":"journal-article","created":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T07:01:21Z","timestamp":1625122881000},"page":"183-195","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Effects of dipyrone and acetylsalicylic acid on contractions of distal cauda epididymis duct, serum testosterone and sperm count in rats"],"prefix":"10.1111","volume":"129","author":[{"given":"Ana Beatriz Melo","family":"Martins","sequence":"first","affiliation":[{"name":"Mode of Drug Action Laboratory Federal University of Rio Grande do Norte  Natal Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayara 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