{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T04:05:10Z","timestamp":1781323510701,"version":"3.54.1"},"reference-count":36,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,9,25]],"date-time":"2021-09-25T00:00:00Z","timestamp":1632528000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100011665","name":"Deanship of Scientific Research, King Saud University","doi-asserted-by":"publisher","award":["J:12\u2010248\u20101440 (HAA)"],"award-info":[{"award-number":["J:12\u2010248\u20101440 (HAA)"]}],"id":[{"id":"10.13039\/501100011665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Basic Clin Pharma Tox"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have investigated the interaction of \u03b1<jats:sub>1<\/jats:sub>\u2010 and \u03b1<jats:sub>2<\/jats:sub>\u2010adrenoceptor subtypes in producing isometric contractions to NA in mouse whole spleen. The \u03b1<jats:sub>1<\/jats:sub>\u2010adrenoceptor antagonist prazosin (10<jats:sup>\u22128<\/jats:sup>\u00a0M) or the \u03b1<jats:sub>2<\/jats:sub>\u2010adrenoceptor antagonist yohimbine (10<jats:sup>\u22126<\/jats:sup>\u00a0M) alone produced only small shifts in NA potency in wild type (WT) mice, but the combination produced a large shift in NA potency. In spleen from \u03b1<jats:sub>1A\/D<\/jats:sub>\u2010KO mice, the effects of prazosin and the combination of prazosin and yohimbine were similar to their effects in WT mice. Hence, in \u03b1<jats:sub>1A\/D<\/jats:sub>\u2010KO mice, in which the only \u03b1<jats:sub>1<\/jats:sub>\u2010adrenoceptor present is the \u03b1<jats:sub>1B<\/jats:sub>\u2010adrenoceptor, prazosin still antagonized contractions to NA. The \u03b1<jats:sub>1A<\/jats:sub>\u2010adrenoceptor antagonist RS100329 (3\u2009\u00d7\u200910<jats:sup>\u22129<\/jats:sup>\u2009M) produced significant shifts in the effects of higher concentrations of NA (EC<jats:sub>50<\/jats:sub> and EC<jats:sub>75<\/jats:sub> levels) and the \u03b1<jats:sub>1D<\/jats:sub>\u2010adrenoceptor antagonist BMY7378 (3\u2009\u00d7\u200910<jats:sup>\u22128<\/jats:sup>\u2009M) produced significant shifts in the effects of lower concentrations of NA (EC<jats:sub>25<\/jats:sub> and EC<jats:sub>50<\/jats:sub> levels). The effects of BMY7378 and RS00329 demonstrate \u03b1<jats:sub>1D<\/jats:sub>\u2010adrenoceptor and \u03b1<jats:sub>1A<\/jats:sub>\u2010adrenoceptor components and suggest that the \u03b1<jats:sub>1B<\/jats:sub>\u2010adrenoceptor interacts with an \u03b1<jats:sub>1D<\/jats:sub>\u2010adrenoceptor, and to a lesser extent an \u03b1<jats:sub>1A<\/jats:sub>\u2010adrenoceptor, at low, and an \u03b1<jats:sub>1A<\/jats:sub>\u2010adrenoceptor at high, NA concentrations. This study demonstrates the complex interaction between \u03b1<jats:sub>1<\/jats:sub>\u2010 and \u03b1<jats:sub>2<\/jats:sub>\u2010adrenoceptor subtypes in producing contractions of mouse spleen and may have general implications for \u03b1\u2010adrenoceptor mediated control of smooth muscle.<\/jats:p>","DOI":"10.1111\/bcpt.13639","type":"journal-article","created":{"date-parts":[[2021,8,12]],"date-time":"2021-08-12T19:15:11Z","timestamp":1628795711000},"page":"416-426","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Interaction between \u03b1<sub>1B<\/sub>\u2010 and other \u03b1<sub>1<\/sub>\u2212 and \u03b1<sub>2<\/sub>\u2010adrenoceptors in producing contractions of mouse spleen"],"prefix":"10.1111","volume":"129","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5379-1385","authenticated-orcid":false,"given":"Hadeel A.","family":"Alsufyani","sequence":"first","affiliation":[{"name":"Department of Physiology 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