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Methamphetamine reduces DAT\u2010mediated dopamine uptake and stimulates dopamine efflux leading to increased synaptic dopamine levels many folds above baseline. Methamphetamine also targets DAT\u2010expressing peripheral immune cells, reduces wound healing and increases infection susceptibility. Peripheral immune cells such as myeloid cells, B cells and T cells express DAT. DAT activity on monocytes and macrophages exhibits immune suppressive properties via an autocrine paracrine mechanism, where deletion or inhibition of DAT activity increases inflammatory responses. In this study, utilizing a mouse model of daily single dose of methamphetamine administration, we investigated the impact of the drug on DAT expression in peripheral immune cells. We found in methamphetamine\u2010treated mice that DAT expression was down\u2010regulated in most of the innate and adaptive immune cells. Methamphetamine did not increase or decrease the total number of innate and adaptive immune cells but changed their immunophenotype to low\u2010DAT\u2010expressing phenotype. Moreover, serum cytokine distributions were altered in methamphetamine\u2010treated mice. Therefore, resembling its effect in the CNS, in the periphery, methamphetamine regulates DAT expression on peripheral immune cell subsets, potentially describing methamphetamine regulation of peripheral immunity.<\/jats:p>","DOI":"10.1111\/bcpt.13838","type":"journal-article","created":{"date-parts":[[2023,1,30]],"date-time":"2023-01-30T01:54:21Z","timestamp":1675043661000},"page":"496-507","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Methamphetamine induces a low dopamine transporter expressing state without altering the total number of peripheral immune cells"],"prefix":"10.1111","volume":"133","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6674-2301","authenticated-orcid":false,"given":"Adithya","family":"Gopinath","sequence":"first","affiliation":[{"name":"Department of Neuroscience University of Florida College of Medicine  Gainesville Florida 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