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Currently, static well\u2010plate assays or manual perfusion systems are used to characterize the interaction of psychostimulants, antidepressants and drugs of abuse with the transporters but still suffer from significant drawbacks caused by lack of automation, for example, low reproducibility, non\u2010comparability of results. An automated microfluidic platform was developed to address the need for more standardized procedures for cell\u2010based assays. An automated system was used to control and drive the simultaneous perfusion of 12 channels on a microfluidic chip, establishing a more standardized protocol to perform release assays to study monoamine transporter\u2010mediated substrate efflux. D\u2010Amphetamine, GBR12909 (norepinephrine transporter) and<jats:italic>p<\/jats:italic>\u2010chloroamphetamine, paroxetine (serotonin transporter) were used as control compounds to validate the system. The platform was able to produce the expected releasing (D\u2010Amphetamine,<jats:italic>p<\/jats:italic>\u2010chloroamphetamine) or inhibiting (GBR12909, paroxetine) profiles for the two transporters. The reduction of manual operation and introduction of automated flow control enabled the implementation of stronger standardized protocols and the possibility of obtaining higher throughput by increasing parallelization.<\/jats:p>","DOI":"10.1111\/bcpt.13940","type":"journal-article","created":{"date-parts":[[2023,9,2]],"date-time":"2023-09-02T05:29:47Z","timestamp":1693632587000},"page":"535-547","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Development and validation of an automated microfluidic perfusion platform for parallelized screening of compounds in vitro"],"prefix":"10.1111","volume":"133","author":[{"given":"Francesca R.","family":"Brugnoli","sequence":"first","affiliation":[{"name":"Elvesys \u2010 Microfluidic Innovation Center Paris France"},{"name":"Center for Physiology and Pharmacology, Institute of Pharmacology Medical University of Vienna Vienna 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