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Ginsenoside Rb1 (GRb1) shows potential hepatoprotective effects. Nonetheless, the protective effect and underlying mechanism of GRb1 against CTD\u2010induced hepatotoxicity in mice have not been investigated. This study aims to elucidate the effect and mechanism of GRb1 on CTD\u2010induced hepatotoxicity using network pharmacology and in vivo experiments. Network pharmacology studies have shown that 263 targets\u00a0were the main mechanisms by which GRb1 alleviates CTD\u2010induced hepatotoxicity. KEGG enrichment analysis revealed that 75 hub genes were mainly enriched in TNF, IL\u201017 and apoptosis signalling pathways. Molecular docking analysis showed that GRb1 exhibited high affinity with Akt1, Tnf, Il6, Bcl2 and Caspase3. In addition, results from animal studies demonstrated that GRb1 could ameliorate CTD\u2010induced hepatotoxicity by inhibiting protein expression of Caspase\u20103, Caspase\u20108, Bcl\u20102\/Bax, GRP78, ATF6, ATF4, CHOP, IRE1\u03b1 and PERK. 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