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The present study aimed to investigate the effect of reactive oxygen species (ROS) accumulation on AFB1\u2010induced mitophagy in HepG2 cells to provide a new perspective from which to design novel therapeutic strategies to treat AFB1 poisoning. ROS release was induced in HepG2 cells with AFB1 (10\u00a0\u03bcmol\/L). Cell autophagy activity, mitochondrial membrane potential (MMP), adenosine triphosphate (ATP) levels, Parkin translocation and both the transcription and expression of mitophagy\u2010related proteins were measured when N\u2010acetyl\u2010L\u2010cysteine (NAC) partially decreased the ROS level, while the knockdown of nuclear factor erythroid 2\u2010related factor 2 (Nrf2) resulted in a large accumulation of ROS. The results reveal that NAC pretreatment ameliorated the decline in both the MMP and the ATP levels while also activating phosphoglycerate mutase 5 (PGAM5)\u2010PTEN\u2010induced kinase 1 (PINK1)\/Parkin, while the Nrf2 knockdown group exhibited the opposite trend. These results suggest that AFB1\u2010induced mitophagy in HepG2 cells depends on ROS, and proper ROS activates mitophagy to play a protective role.<\/jats:p>","DOI":"10.1111\/bcpt.14034","type":"journal-article","created":{"date-parts":[[2024,5,28]],"date-time":"2024-05-28T05:06:10Z","timestamp":1716872770000},"page":"195-209","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Aflatoxin B1 induces ROS\u2010dependent mitophagy by modulating the PINK1\/Parkin pathway in HepG2 cells"],"prefix":"10.1111","volume":"135","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-2771-452X","authenticated-orcid":false,"given":"Yuxi","family":"Wang","sequence":"first","affiliation":[{"name":"Institute of Public Health Chengdu University of Traditional Chinese Medicine  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lan","family":"Long","sequence":"additional","affiliation":[{"name":"Deyang Center for Disease Control and Prevention  Deyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Luo","sequence":"additional","affiliation":[{"name":"Institute of Public Health Chengdu University of Traditional Chinese Medicine  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyi","family":"Huang","sequence":"additional","affiliation":[{"name":"Institute of Public Health Chengdu University of Traditional Chinese Medicine  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Public Health Chengdu University of Traditional Chinese Medicine  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Meng","sequence":"additional","affiliation":[{"name":"Institute of Public Health Chengdu University of Traditional Chinese Medicine  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dayi","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Public Health Chengdu University of Traditional Chinese Medicine  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,5,28]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2021.108120"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12550\u2010016\u20100265\u20107"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2019.1658570"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms222413322"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/19440049.2011.563370"},{"key":"e_1_2_8_7_1","unstructured":"IARC.Chemical agents and related occupations: a review of human carcinogens. 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