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C57BL\/6 mice were intraperitoneally administered KM (0.8, 2.4 or 7.2\u00a0mg\/kg), colchicine (3.0\u00a0mg\/kg) or sterile saline. One hour later, a monosodium urate (MSU) suspension was injected into the right hind paws of the mice to establish an acute gout model. Inflammation symptoms were evaluated at 0, 3, 6, 12 and 24\u2009h, and the mechanical withdrawal threshold was evaluated at 0, 6 and 24\u2009h. After 24\u2009h, the mice were euthanized, and the joint tissue, kidney and blood were collected for subsequent experiments. Histological examination and antioxidant enzyme, kidney index and serum uric acid (UA) measurements were taken. The expression levels of the signalling pathway components were determined. KM effectively alleviated the symptoms of redness, swelling and pain; counteracted inflammatory cell infiltration; and increased antioxidant enzyme levels, reduced kidney index and serum UA levels through regulating UA excretion in MSU\u2010induced mice. The expression of toll\u2010like receptor 4 (TLR4)\/nuclear factor kappa\u2010B (NF\u2010\u03baB)\/nucleotide\u2010binding oligomerization domain, leucine\u2010rich repeat and pyrin domain\u2010containing 3 (NLRP3) signalling pathway proteins and mRNA were reduced in the KM group. These results suggest that KM may be effective in alleviating GA through the TLR4\/NF\u2010\u03baB\/NLRP3 pathway.<\/jats:p>","DOI":"10.1111\/bcpt.14037","type":"journal-article","created":{"date-parts":[[2024,6,3]],"date-time":"2024-06-03T10:29:16Z","timestamp":1717410556000},"page":"133-147","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The alleviatory effects of koumine on MSU\u2010induced gouty arthritis via the TLR4\/NF\u2010\u03baB\/NLRP3 pathway"],"prefix":"10.1111","volume":"135","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-9063-2843","authenticated-orcid":false,"given":"Shi\u2010kang","family":"Lin","sequence":"first","affiliation":[{"name":"School of Stomatology Fujian Medical University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shi\u2010ting","family":"Chen","sequence":"additional","affiliation":[{"name":"Fujian Key Laboratory of Natural Medicine Pharmacology, 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