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Ascorbic acid was used as an antioxidative agent to stabilize olanzapine during storage and sample preparation.<\/jats:p><jats:p>We assessed analyte stability on long\u2010term storage in serum samples at 25\u00b0C, 5\u00b0C, \u221220\u00b0C and \u221280\u00b0C, and during five freeze\u2013thaw cycles. Analytes were stable for 23\u2009days at room temperature except for olanzapine and mirtazapine (17\u2009days). All analytes were stable for at least 30\u2009days at 5\u00b0C. All analytes were stable for 270\u2009days at \u221220\u00b0C, except for paliperidone and mirtazapine with 60\u2009days and 180 days, respectively. All analytes were stable for 270\u2009days at \u221280\u00b0C. Furthermore, all analytes were stable for five freeze\u2013thaw cycles.<\/jats:p><jats:p>We recommend storage at \u221280\u00b0C when samples drawn for analysis of antipsychotic drugs are stored for more than 60\u2009days, whereas a temperature of \u221220\u00b0C is sufficient for storage less than 60\u2009days.<\/jats:p>","DOI":"10.1111\/bcpt.14080","type":"journal-article","created":{"date-parts":[[2024,9,24]],"date-time":"2024-09-24T06:49:25Z","timestamp":1727160565000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Long\u2010term stability of five atypical antipsychotics (risperidone, olanzapine, paliperidone, clozapine, quetiapine) and the antidepressant mirtazapine in human serum assessed by a validated SPE LC\u2013MS\/MS method"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7274-8486","authenticated-orcid":false,"given":"Palle Bach Nielsen","family":"Fruekilde","sequence":"first","affiliation":[{"name":"Dept. of Clinical Biochemistry Odense University Hospital  Odense Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flemming","family":"Nielsen","sequence":"additional","affiliation":[{"name":"Clinical Pharmacology, Pharmacy and Environmental Medicine. 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