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In this study, we deciphered the key mechanism by which DATS alleviates ulcerative colitis (UC). We showed that oral administration of DATS for 10 consecutive days greatly restrained the infiltration of macrophages and the pathological changes in colonic tissues of mice with DSS\u2010induced colitis. DATS treatment notably dampened the content of IL\u20101\u03b2 and IL\u201018 and suppressed NLRP3 inflammasome activation in colon. Mechanistically, DATS effectively diminished the generation of ROS in macrophages. The suppressive effect of DATS on the activation of NLRP3 inflammasome and downregulation of IL\u201018 and IL\u20101\u03b2 levels was blunted by xanthine oxidase. Further studies revealed that DATS inhibited NF\u2010\u03baB pathway activation by suppressing the expression of Trx\u20101, thereby inhibiting NLRP3 inflammasome activation. Trx\u20101 overexpression and interference in macrophages promoted and diminished NLRP3 inflammasome activation, respectively. In summary, garlic and its main active ingredient DATS have potentials to prevent and treat UC, and DATS functions by inhibiting NLRP3 inflammasome activation via Trx\u20101\/ROS pathway.<\/jats:p>","DOI":"10.1111\/bcpt.14083","type":"journal-article","created":{"date-parts":[[2024,9,26]],"date-time":"2024-09-26T08:59:57Z","timestamp":1727341197000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Diallyl trisulfide alleviates dextran sulphate sodium\u2010induced colitis in mice by inhibiting NLRP3 inflammasome activation via ROS\/Trx\u20101 pathway"],"prefix":"10.1111","author":[{"given":"Yue","family":"He","sequence":"first","affiliation":[{"name":"Department of Pharmacology of Chinese Materia Medica, School of Traditional Chinese Pharmacy China Pharmaceutical University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Pharmacology of Chinese Materia Medica, School of Traditional Chinese Pharmacy China Pharmaceutical University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, School of Traditional Chinese Pharmacy China Pharmaceutical University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanrong","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology of Chinese Materia Medica, School of Traditional Chinese Pharmacy China Pharmaceutical University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yilei","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Pharmacology of Chinese Materia Medica, School of Traditional Chinese Pharmacy China Pharmaceutical University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yufeng","family":"Xia","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, School of Traditional Chinese Pharmacy China Pharmaceutical University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huatou","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Cardiology Nanjing Gaochun People's Hospital  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhifeng","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Pharmacology of Chinese Materia Medica, School of Traditional Chinese Pharmacy China Pharmaceutical University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4872-2064","authenticated-orcid":false,"given":"Yue","family":"Dai","sequence":"additional","affiliation":[{"name":"Department of Pharmacology of Chinese Materia Medica, School of Traditional Chinese Pharmacy China Pharmaceutical University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,9,26]]},"reference":[{"doi-asserted-by":"crossref","unstructured":"UngaroR MehandruS AllenPB et al.Ulcerative colitis. 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